Cytotoxic and Antiproliferative Effects of Diarylheptanoids Isolated from Curcuma comosa Rhizomes on Leukaemic Cells

• Published in: Molecules (Basel, Switzerland) Switzerland), 2020-11, Vol.25 (22), p.5476
• Main Authors: Viriyaadhammaa, Natsima, Saiai, Aroonchai, Neimkhum, Waranya, Nirachonkul, Wariya, Chaiyana, Wantida, Chiampanichayakul, Sawitree, Tima, Singkome, Usuki, Toyonobu, Duangmano, Suwit, Anuchapreeda, Songyot
• Format: Article
• Language: English
• Subjects: Animals; anti-inflammatory; Anti-inflammatory agents; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; antioxidant; Antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Antiproliferatives; Apoptosis; Aquatic plants; Ascorbic acid; Blood; Cancer; Cell cycle; Cell Cycle - drug effects; Cell growth; Cell Line, Tumor; Cell proliferation; Cell Proliferation - drug effects; Chromatography; Chromatography - methods; Curcuma; Curcuma - chemistry; Curcuma comosa; Cytotoxicity; diarylheptanoids; Diarylheptanoids - chemistry; Diarylheptanoids - isolation & purification; Diarylheptanoids - pharmacology; Dose-Response Relationship, Drug; Erythrocytes; Flow Cytometry; Gene Expression; Hemolysis; Humans; Inflammation; Leukemia; Leukemia - genetics; Leukemia - metabolism; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - metabolism; Menstruation; Mice; Molecular Structure; NMR; Nuclear magnetic resonance; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Protein expression; Proteins; RAW 264.7 Cells; Rhizome - chemistry; Rhizomes; S phase; Tumors; Urogenital system; WT1 protein; WT1 Proteins - genetics; WT1 Proteins - metabolism; Zingiberaceae
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25225476

belongs to the Zingiberaceae family. In this study, two natural compounds were isolated from , and their structures were determined using nuclear magnetic resonance. The isolated compounds were identified as 7-(3,4-dihydroxyphenyl)-5-hydroxy-1-phenyl-(1 )-1-heptene ( ) and -1,7-diphenyl-5-hydroxy-1-heptene ( ). Compound showed the strongest cytotoxicity effect against HL-60 cells, while its antioxidant and anti-inflammatory properties were stronger than those of compound . Compound proved to be a potent antioxidant, compared to ascorbic acid. Neither compounds had any effect on red blood cell haemolysis. Furthermore, compound significantly decreased Wilms' tumour 1 protein expression and cell proliferation in KG-1a cells. Compound decreased the WT1 protein levels in a time- and dose- dependent manner. Compound suppressed cell cycle at the S phase. In conclusion, compound has a promising chemotherapeutic potential against leukaemia.