Probiotics Enhance Bone Growth and Rescue BMP Inhibition: New Transgenic Zebrafish Lines to Study Bone Health

Zebrafish larvae, especially gene-specific mutants and transgenic lines, are increasingly used to study vertebrate skeletal development and human pathologies such as osteoporosis, osteopetrosis and osteoarthritis. Probiotics have been recognized in recent years as a prophylactic treatment for variou...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-04, Vol.23 (9), p.4748
Main Authors: Sojan, Jerry Maria, Raman, Ratish, Muller, Marc, Carnevali, Oliana, Renn, Jörg
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Artificial chromosomes
Bacillus subtilis
Biochemistry, biophysics & molecular biology
Biochimie, biophysique & biologie moléculaire
Biomedical materials
BMP inhibitors
Bone Density
Bone Development
Bone growth
Bone matrix
Bones
Cbfa-1 protein
Danio rerio
Embryos
Extracellular matrix
Fluorescence
Gene expression
Genes
Larva - genetics
Larvae
Life sciences
Microbiota
Mineralization
Osteoarthritis
Osteoblasts
Osteoblasts - metabolism
Osteopetrosis
Osteoporosis
Probiotics
Probiotics - pharmacology
Proteins
Sciences du vivant
transgenic lines
Vertebrates
Zebrafish
Zebrafish - genetics
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Summary:Zebrafish larvae, especially gene-specific mutants and transgenic lines, are increasingly used to study vertebrate skeletal development and human pathologies such as osteoporosis, osteopetrosis and osteoarthritis. Probiotics have been recognized in recent years as a prophylactic treatment for various bone health issues in humans. Here, we present two new zebrafish transgenic lines containing the coding sequences for fluorescent proteins inserted into the endogenous genes for and with larvae displaying fluorescence in developing osteoblasts and the bone extracellular matrix (mineralized or non-mineralized), respectively. Furthermore, we use these transgenic lines to show that exposure to two different probiotics, and , leads to an increase in osteoblast formation and bone matrix growth and mineralization. Gene expression analysis revealed the effect of the probiotics, particularly , in modulating several skeletal development genes, such as , , and , further supporting their ability to improve bone health. was the more potent probiotic able to significantly reverse the inhibition of bone matrix formation when larvae were exposed to a BMP inhibitor (LDN212854).
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23094748