Comprehensive In Silico Analysis and Transcriptional Profiles Highlight the Importance of Mitochondrial Dicarboxylate Carriers (DICs) on Hypoxia Response in Both Arabidopsis thaliana and Eucalyptus grandis

Plant dicarboxylate carriers (DICs) transport a wide range of dicarboxylates across the mitochondrial inner membrane. The family is composed of three genes ( , and ), whereas two genes ( and ) have been retrieved in . Here, by combining in silico and analyses, we provide evidence that DICs are parti...

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Bibliographic Details
Published in:Plants (Basel) 2022-01, Vol.11 (2), p.181
Main Authors: Barreto, Pedro, Arcuri, Mariana L C, Lima, Rômulo Pedro Macêdo, Marino, Celso Luis, Maia, Ivan G
Format: Article
Language:English
Subjects:
abiotic stress
Amino acids
Arabidopsis
Arabidopsis thaliana
Binding sites
DIC
dicarboxylates
Environmental stress
Eucalyptus
Eucalyptus grandis
Flowers & plants
Gene expression
Genes
Genomes
Hypoxia
Metabolism
Mitochondria
Oxygen
Plant mitochondria
Plant species
Proteins
Seedlings
Starvation
Stresses
Submergence
Transcription factors
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Summary:Plant dicarboxylate carriers (DICs) transport a wide range of dicarboxylates across the mitochondrial inner membrane. The family is composed of three genes ( , and ), whereas two genes ( and ) have been retrieved in . Here, by combining in silico and analyses, we provide evidence that DICs are partially redundant, important in plant adaptation to environmental stresses and part of a low-oxygen response in both species. and are present in most plant species and have very similar gene structure, developmental expression patterns and absolute expression across natural Arabidopsis accessions. In contrast, seems to be an early genome acquisition found in Brassicaceae and shows relatively low (or no) expression across these accessions. In silico analysis revealed that both and are highly responsive to stresses, especially to cold and submergence, while their promoters are enriched for stress-responsive transcription factors binding sites. The expression of and is highly correlated across natural accessions and in response to stresses, while no correlation was found for . Gene ontology enrichment analysis suggests a role for and in response to hypoxia, and for in phosphate starvation. Accordingly, the investigated genes are induced by submergence stress in and while overexpression improved seedling survival to submergence. Interestingly, the induction of and is abrogated in the mutant that is devoid of plant oxygen sensing, suggesting that these genes are part of a conserved hypoxia response in Arabidopsis.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11020181