Circulating extracellular vesicles as novel biomarkers for pulmonary arterial hypertension in patients with systemic lupus erythematosus

• Published in: Frontiers in immunology 2024-09, Vol.15, p.1374100
• Main Authors: Ding, Zhe, Qi, Fumin, Liu, Li, Wang, Zhouming, Zhang, Na, Lyu, Xing, Sun, Wenwen, Du, Jun, Song, Haoming, Hou, Hou, Guo, Ying, Wang, Xiaomei, Liu, Ming-Lin, Wei, Wei
• Format: Article
• Language: English
• Subjects: Adult; Annexin A5 - blood; biomarker; Biomarkers - blood; Case-Control Studies; Endothelial Cells - metabolism; extracellular vesicles; Extracellular Vesicles - metabolism; Female; Humans; Hypertension, Pulmonary - blood; Hypertension, Pulmonary - diagnosis; Hypertension, Pulmonary - etiology; Immunology; Lupus Erythematosus, Systemic - blood; Lupus Erythematosus, Systemic - complications; Male; Middle Aged; procoagulant; pulmonary arterial hypertension; Pulmonary Arterial Hypertension - blood; Pulmonary Arterial Hypertension - diagnosis; Pulmonary Arterial Hypertension - etiology; systemic lupus erythematosus
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2024.1374100

Pulmonary arterial hypertension (PAH) is a serious complication of systemic lupus erythematosus (SLE) with increased mortality. A prothrombotic state may contribute to pathogenesis of SLE-PAH. Extracellular vesicles (EVs) are known to be associated with thrombosis. Here, we investigated circulating EVs and their associations with SLE-PAH. Eighteen SLE-PAH patients, 36 SLE-non-PAH patients, and 36 healthy controls (HCs) were enrolled. Flow cytometry was used to analyze circulating EVs from leukocytes (LEVs), red blood cells (REVs), platelets (PEVs), endothelial cells (EEVs), and Annexin V EVs with membrane phosphatidylserine (PS) exposure. Plasma levels of all EV subgroups were elevated in SLE patients with or without PAH compared to HCs. Furthermore, plasma Annexin V EVs, LEVs, PEVs, REVs, EEVs, and Annexin V REVs were significantly elevated in SLE-PAH patients compared to SLE-non-PAH patients. Additionally, PAH patients with moderate/high SLE showed a significant increase in LEVs, PEVs, REVs, Annexin V EVs, and Annexin V REVs compared to SLE-non-PAH patients. However, PAH patients with inactive/mild SLE only exhibited elevations in Annexin V EVs, REVs, and Annexin V REVs. In the SLE-PAH patients, EEVs were positively correlated with pulmonary arterial systolic pressure, while PEVs and EEVs were positively correlated with right ventricular diameter. Moreover, the receiver operating characteristic curve indicated that Annexin V EVs, LEVs, PEVs, REVs, EEVs and Annexin V REVs could predict the presence of PAH in SLE patients. Importantly, multivariate logistic regression analysis showed that circulating levels of LEVs or REVs, anti-nRNP antibody, and serositis were independent risk factors for PAH in SLE patients. Findings reveal that specific subgroups of circulating EVs contribute to the hypercoagulation state and the severity of SLE-PAH. Higher plasma levels of LEVs or REVs may serve as biomarkers for SLE-PAH.