Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine

Apicomplexan parasites, including Toxoplasma gondii (T. gondii), express different types of calcium-dependent protein kinases (CDPKs), which perform a variety of functions, including attacking and exiting the host cells. In the current bioinformatics study, we have used several web servers to predic...

Full description

Saved in:
Bibliographic Details
Published in:Journal of parasitology research 2021, Vol.2021, p.9974509-20
Main Authors: Taghipour, Ali, Tavakoli, Sanaz, Sabaghan, Mohamad, Foroutan, Masoud, Majidiani, Hamidreza, Soltani, Shahrzad, Badri, Milad, Ghaffari, Ali Dalir, Soltani, Sheyda
Format: Article
Language:English
Subjects:
Analysis
Animal models in research
Care and treatment
Computational biology
Development and progression
Diagnosis
Genetic aspects
Health aspects
Immunological research
Parasite vaccines
Prevention
Protein kinases
Risk factors
Toxoplasmosis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Apicomplexan parasites, including Toxoplasma gondii (T. gondii), express different types of calcium-dependent protein kinases (CDPKs), which perform a variety of functions, including attacking and exiting the host cells. In the current bioinformatics study, we have used several web servers to predict the basic features and specifications of the CDPK7 protein. The findings showed that CDPK7 protein has 2133 amino acid residues with an average molecular weight (MW) of 219085.79 D. The aliphatic index with 68.78 and grand average of hydropathicity (GRAVY) with -0.331 score were estimated. The outcomes of current research showed that the CDPK7 protein included 502 alpha-helix, 1311 random coils, and 320 extended strands with GOR4 method. Considering the Ramachandran plot, the favored region contains more than 92% of the amino acid residues. In addition, evaluation of antigenicity and allergenicity showed that CDPK7 protein has immunogenic and nonallergenic nature. The present research provides key data for more animal-model study on the CDPK7 protein to design an efficient vaccine against toxoplasmosis in the future.
ISSN:2090-0023
2090-0031
DOI:10.1155/2021/9974509