Genome-Wide Identification, Classification, Expression Analysis, and Screening of Drought and Heat Resistance-Related Candidates of the Rboh Gene Family in Wheat

Plant respiratory burst oxidase homologs (Rbohs) are key enzymes that produce reactive oxygen species (ROS), which serve as signaling molecules regulating plant growth and stress responses. In this study, 39 genes ( - ) were identified. These genes were distributed unevenly among the wheat genome�...

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Bibliographic Details
Published in:Plants (Basel) 2024-12, Vol.13 (23), p.3377
Main Authors: Cao, Miyuan, Zhang, Yue, Zou, Xiaoxiao, Yin, Huangping, Yin, Yan, Li, Zeqi, Xiao, Wenjun, Liu, Shucan, Li, Yongliang, Guo, Xinhong
Format: Article
Language:English
Subjects:
abiotic stress
Binding sites
Cell cycle
Cellular stress response
Chromosomes
Drought
Evolutionary conservation
Evolutionary genetics
expression patterns
Food supply
Gene expression
Genes
Genomes
Genomic analysis
Genomics
Heat resistance
Hormones
Identification
Localization
Oxidases
Phylogenetics
Plant growth
Proteins
Rbohs
Reactive oxygen species
Respiratory burst oxidase
Signal transduction
Stresses
Thermal resistance
Triticum aestivum
Wheat
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Summary:Plant respiratory burst oxidase homologs (Rbohs) are key enzymes that produce reactive oxygen species (ROS), which serve as signaling molecules regulating plant growth and stress responses. In this study, 39 genes ( - ) were identified. These genes were distributed unevenly among the wheat genome's fourteen chromosomes, with the exception of homoeologous group 2 and 7 and chromosomes 4A, as well as one unidentified linkage group (Un). TaRbohs were classified into ten distinct clades, each sharing similar motif compositions and gene structures. The promoter regions of s contained -elements related to hormones, growth and development, and stresses. Furthermore, five genes ( , , , , and ) exhibited strong evolutionary conservation. Additionally, a Ka/Ks analysis confirmed that purifying selection was the predominant force driving the evolution of these genes. Expression profiling and qPCR results further indicated differential expression patterns of genes between heat and drought stresses. , , , , , and were significantly upregulated under multiple stress conditions, whereas was only elevated in response to drought stress. Collectively, our findings provide a systematic analysis of the wheat gene family and establish a theoretical framework for our future research on the role of genes in response to heat and drought stress.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants13233377