Heterotrophic Selenium Incorporation into Chlorella vulgaris K-01: Selenium Tolerance, Assimilation, and Removal through Microalgal Cells

• Published in: Foods 2024-01, Vol.13 (3), p.405
• Main Authors: Zhang, Zhenyu, Zhang, Yan, Hua, Yanying, Chen, Guancheng, Fu, Pengcheng, Liu, Jing
• Format: Article
• Language: English
• Subjects: Algae; Aquatic microorganisms; Bioavailability; Biomass; Biotransformation; Carbon; Carrying capacity; Cell culture; Chlorella; Chlorella vulgaris; Dose-response effects; Electron microscopes; Energy metabolism; Functional foods & nutraceuticals; Gaussian process; Glucose; Growth curves; heterotrophic cultivation; Hypotheses; Mass spectrometry; Mass spectroscopy; Metabolic response; Metabolism; Microalgae; organic selenium; Productivity; Regression analysis; Scanning electron microscopy; Selenium; Strain
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods13030405

has been applied in the production of selenium (Se) enriched organic biomass. However, limited information exists regarding heterotrophic selenium tolerance and its incorporation into . This study aimed to investigate the potential of using K-01 for selenium biotransformation. To assess the dose-response effect of Se stress on the strain, time-series growth curves were recorded, growth productivity parameters were calculated, and Gaussian process (GP) regression analysis was performed. The strain's carbon and energy metabolism were evaluated by measuring residual glucose in the medium. Characterization of different forms of intracellular Se and residual Se in the medium was conducted using inductively coupled plasma-mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometer (ICP-OES). The EC50 value for the strain in response to Se stress was 38.08 mg/L. The maximum biomass productivity was 0.26 g/L/d. GP regression analysis revealed that low-level Se treatment could increase the biomass accumulation and the carrying capacity of K-01 in a heterotrophic culture. The maximum organic Se in biomass was 154.00 μg/g DW. These findings lay the groundwork for understanding heterotrophic microalgal production of Se-containing nutraceuticals, offering valuable insights into Se tolerance, growth dynamics, and metabolic responses in K-01.