Arabidopsis 14-3-3 epsilon members contribute to polarity of PIN auxin carrier and auxin transport-related development

Eukaryotic 14-3-3 proteins have been implicated in the regulation of diverse biological processes by phosphorylation-dependent protein-protein interactions. The genome encodes two groups of 14-3-3s, one of which - epsilon - is thought to fulfill conserved cellular functions. Here, we assessed the in...

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Bibliographic Details
Published in:eLife 2017-04, Vol.6
Main Authors: Keicher, Jutta, Jaspert, Nina, Weckermann, Katrin, Möller, Claudia, Throm, Christian, Kintzi, Aaron, Oecking, Claudia
Format: Article
Language:English
Subjects:
14-3-3
14-3-3 protein
14-3-3 Proteins - genetics
14-3-3 Proteins - metabolism
Arabidopsis
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - metabolism
auxin transport
Deoxyribonucleic acid
DNA
Flowers & plants
Gene Silencing
Gene Targeting
Genomes
Golgi apparatus
Indoleacetic Acids - metabolism
Membrane trafficking
Membrane Transport Proteins - metabolism
miRNA
Oryza sativa
Phosphorylation
Phylogenetics
Physcomitrella patens
PIN polarity
Plant Biology
Plant Development
Plant Growth Regulators - metabolism
Polarity
Protein interaction
Proteins
RNA-mediated interference
Seedlings
Software
Solanum lycopersicum
Trees
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Summary:Eukaryotic 14-3-3 proteins have been implicated in the regulation of diverse biological processes by phosphorylation-dependent protein-protein interactions. The genome encodes two groups of 14-3-3s, one of which - epsilon - is thought to fulfill conserved cellular functions. Here, we assessed the in vivo role of the ancestral 14-3-3 epsilon group members. Their simultaneous and conditional repression by RNA interference and artificial microRNA in seedlings led to altered distribution patterns of the phytohormone auxin and associated auxin transport-related phenotypes, such as agravitropic growth. Moreover, 14-3-3 epsilon members were required for pronounced polar distribution of PIN-FORMED auxin efflux carriers within the plasma membrane. Defects in defined post-Golgi trafficking processes proved causal for this phenotype and might be due to lack of direct 14-3-3 interactions with factors crucial for membrane trafficking. Taken together, our data demonstrate a fundamental role for the ancient 14-3-3 epsilon group members in regulating PIN polarity and plant development.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.24336