NRF2 deficiency replicates transcriptomic changes in Alzheimer's patients and worsens APP and TAU pathology

• Published in: Redox biology 2017-10, Vol.13 (C), p.444-451
• Main Authors: Rojo, Ana I., Pajares, Marta, Rada, Patricia, Nuñez, Angel, Nevado-Holgado, Alejo J., Killik, Richard, Van Leuven, Fred, Ribe, Elena, Lovestone, Simon, Yamamoto, Masayuki, Cuadrado, Antonio
• Format: Article
• Language: English
• Subjects: Alzheimer Disease - genetics; Alzheimer Disease - metabolism; Alzheimer Disease - pathology; Alzheimer's disease; Amyloid beta-Protein Precursor - genetics; Amyloid beta-Protein Precursor - metabolism; Animals; Brain - metabolism; Brain - pathology; Brain - physiopathology; Humans; Long-Term Potentiation; Maze Learning; Mice; Mice, Inbred C57BL; Neuroinflammation; NF-E2-Related Factor 2 - deficiency; NF-E2-Related Factor 2 - genetics; NF-E2-Related Factor 2 - metabolism; NRF2; Oxidative Stress; Proteotoxicity; Research Paper; tau Proteins - genetics; tau Proteins - metabolism; Transcriptome; Transcriptomics
• ISSN: 2213-2317; 2213-2317
• DOI: 10.1016/j.redox.2017.07.006

Failure to translate successful neuroprotective preclinical data to a clinical setting in Alzheimer's disease (AD) indicates that amyloidopathy and tauopathy alone provide an incomplete view of disease. We have tested here the relevance of additional homeostatic deviations that result from loss of activity of transcription factor NRF2, a crucial regulator of multiple stress responses whose activity declines with ageing. A transcriptomic analysis demonstrated that NRF2-KO mouse brains reproduce 7 and 10 of the most dysregulated pathways of human ageing and AD brains, respectively. Then, we generated a mouse that combines amyloidopathy and tauopathy with either wild type (AT-NRF2-WT) or NRF2-deficiency (AT-NRF2-KO). AT-NRF2-KO brains presented increased markers of oxidative stress and neuroinflammation as well as higher levels of insoluble phosphorylated-TAU and Aβ*56 compared to AT-NRF2-WT mice. Young adult AT-NRF2-KO mice exhibited deficits in spatial learning and memory and reduced long term potentiation in the perforant pathway. This study demonstrates the relevance of normal homeostatic responses that decline with ageing, such as NRF2 activity, in the protection against proteotoxic, inflammatory and oxidative stress and provide a new strategy to fight AD. [Display omitted] •NRF2-lack mimics many transcriptomic alterations of elderly and AD patients.•APP/TAU-induced oxidative and inflammatory stress is exacerbated by NRF2-deficiency.•APP/TAU-induced proteinopathy is exacerbated by NRF2-deficiency.•APP/TAU-induced deficits in cognition are aggravated by NRF2-deficiency.