Tensile force impairs lip muscle regeneration under the regulation of interleukin‐10

Background Orbicularis oris muscle, the crucial muscle in speaking, facial expression and aesthetics, is considered the driving force for optimal lip repair. Impaired muscle regeneration remains the main culprit for unsatisfactory surgical outcomes. However, there is a lack of study on how different...

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Bibliographic Details
Published in:Journal of cachexia, sarcopenia and muscle sarcopenia and muscle, 2024-12, Vol.15 (6), p.2497-2508
Main Authors: Cheng, Xu, Dou, Jinfeng, Li, Jinggui, Huang, Yixuan, Shi, Bing, Li, Jingtao
Format: Article
Language:English
Subjects:
Animals
Antibodies
Cytokines
Dissection
Fibrosis
inflammation mediators
Interleukin-10 - metabolism
Lip
Male
mesenchymal stromal cells
Muscle Development
Muscle, Skeletal - injuries
Muscle, Skeletal - metabolism
Musculoskeletal system
Myoblasts - metabolism
Original
orofacial cleft
Rats
Regeneration
satellite cells, skeletal muscle
Satellite Cells, Skeletal Muscle - metabolism
surgical procedures, operative
Tensile Strength
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Summary:Background Orbicularis oris muscle, the crucial muscle in speaking, facial expression and aesthetics, is considered the driving force for optimal lip repair. Impaired muscle regeneration remains the main culprit for unsatisfactory surgical outcomes. However, there is a lack of study on how different surgical manipulations affect lip muscle regeneration, limiting efforts to seek effective interventions. Methods In this study, we established a rat lip surgery model where the orbicularis oris muscle was injured by manipulations including dissection, transection and stretch. The effect of each technique on muscle regeneration was examined by histological analysis of myogenesis and fibrogenesis. The impact of tensile force was further investigated by the in vitro application of mechanical strain on cultured myoblasts. Transcriptome profiling of muscle satellite cells from different surgical groups was performed to figure out the key factors mediating muscle fibrosis, followed by therapeutic intervention to improve muscle regeneration after lip surgeries. Results Evaluation of lip muscle regeneration till 56 days after injury revealed that the stretch group resulted in the most severe muscle fibrosis (n = 6, fibrotic area 48.9% in the stretch group, P 
ISSN:2190-5991
2190-6009
2190-6009
DOI:10.1002/jcsm.13584