Resveratrol promotes osteoblastic differentiation in a rat model of postmenopausal osteoporosis by regulating autophagy

Resveratrol is a natural polyphenolic compound that ameliorates postmenopausal osteoporosis by activating the estrogen receptor. Research has shown that resveratrol exhibits some type of estrogen receptor agonist activity, reducing the risk of breast cancer. However, its mechanism of action remains...

Full description

Saved in:
Bibliographic Details
Published in:Nutrition & metabolism 2020-04, Vol.17 (1), p.29-29, Article 29
Main Authors: Wang, Wei, Zhang, Li-Mei, Guo, Chang, Han, Jian-Feng
Format: Article
Language:English
Subjects:
Autophagy
Bone density
Bone marrow
Bone mineral density
Breast cancer
Calcium
Cancer
Cancer therapies
Collagen
Collagen (type I)
Enzymes
Estrogen
Femur
Health aspects
Hormone replacement therapy
Laboratory animals
Leupeptin
Ligands
Oncology, Experimental
Osteoblastogenesis
Osteoblasts
Osteoclastogenesis
Osteoclasts
Osteoporosis
Ovariectomy
Phagocytosis
Post-menopause
Postmenopausal women
Prevention
Resveratrol
Sedimentation & deposition
Tibia
TRANCE protein
Vascular endothelial growth factor
Vertebrae
Western blotting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Resveratrol is a natural polyphenolic compound that ameliorates postmenopausal osteoporosis by activating the estrogen receptor. Research has shown that resveratrol exhibits some type of estrogen receptor agonist activity, reducing the risk of breast cancer. However, its mechanism of action remains largely unknown. This study aims to investigate the effect of resveratrol on osteoblastic and osteoclastic differentiation and its potential role in the regulation of autophagy. Sprague Dawley (SD) rats underwent ovariectomies (OVX) and were administered resveratrol (at 10, 20 or 40 mg/kg/d) for 8 weeks. The calcium content and the bone mineral density (BMD) were measured in the lumbar vertebrae (L3) and the right distal femur-tibia bone region. The osteoblasts and osteoclasts were isolated from rat lumbar vertebrae by enzyme digestion and bone marrow induction, respectively. The cells were then cultured with resveratrol in combination with bafilomycin or leupeptin to inhibit or activate autophagy, respectively. Western blotting was used to assess the differentiation markers and autophagy-related genes in the osteoblasts and osteoclasts. Compared to the sham group, the bone calcium content and BMD were significantly decreased in the OVX group (  
ISSN:1743-7075
1743-7075
DOI:10.1186/s12986-020-00449-9