Loss of Function of Rice Plastidic Glycolate/Glycerate Translocator 1 Impairs Photorespiration and Plant Growth

Ribulose-1,5-bisphosphate carboxylase/oxygenase, the key enzyme of photosynthetic carbon fixation, is able to accept both O and CO as substrates. When it fixes O , it produces 2-phosphoglycolate, which is detoxified by photorespiration and recycled to the Calvin-Benson-Bassham cycle. To complete pho...

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Bibliographic Details
Published in:Frontiers in plant science 2020-01, Vol.10, p.1726
Main Authors: Shim, Su-Hyeon, Lee, Sang-Kyu, Lee, Dae-Woo, Brilhaus, Dominik, Wu, Guangxi, Ko, Sooyeon, Lee, Choon-Hwan, Weber, Andreas P M, Jeon, Jong-Seong
Format: Article
Language:English
Subjects:
OsPLGG1
photorespiration
photosynthesis
Plant Science
plastidic glycolate/glycerate translocator
rice
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Summary:Ribulose-1,5-bisphosphate carboxylase/oxygenase, the key enzyme of photosynthetic carbon fixation, is able to accept both O and CO as substrates. When it fixes O , it produces 2-phosphoglycolate, which is detoxified by photorespiration and recycled to the Calvin-Benson-Bassham cycle. To complete photorespiration, metabolite transport across three organelles, chloroplasts, peroxisomes, and mitochondria, is necessary through transmembrane transporters. In rice ( ) little is known about photorespiratory transmembrane transporters. Here, we identified the rice plastidic glycolate/glycerate translocator 1 (OsPLGG1), a homolog of PLGG1. OsPLGG1 mutant lines, , , and , showed a growth retardation phenotype, such as pale green leaf, reduced tiller number, and reduced seed grain weight as well as reduced photosynthetic carbon reduction rate due to low activities of photosystem I and II. The plant growth retardation in mutants was rescued under high CO condition. Subcellular localization of OsPLGG1-GFP fusion protein, along with its predicted N-terminal transmembrane domain, confirmed that OsPLGG1 is a chloroplast transmembrane protein. Metabolite analysis indicated significant accumulation of photorespiratory metabolites, especially glycolate and glycerate, which have been shown to be transported by the PLGG1, and changes for a number of metabolites which are not intermediates of photorespiration in the mutants. These results suggest that OsPLGG1 is the functional plastidic glycolate/glycerate transporter, which is necessary for photorespiration and growth in rice.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2019.01726