Herbivorous turtle ants obtain essential nutrients from a conserved nitrogen-recycling gut microbiome

• Published in: Nature communications 2018-03, Vol.9 (1), p.964-14, Article 964
• Main Authors: Hu, Yi, Sanders, Jon G., Łukasik, Piotr, D’Amelio, Catherine L., Millar, John S., Vann, David R., Lan, Yemin, Newton, Justin A., Schotanus, Mark, Kronauer, Daniel J. C., Pierce, Naomi E., Moreau, Corrie S., Wertz, John T., Engel, Philipp, Russell, Jacob A.
• Format: Article
• Language: English
• Subjects: 38/22; 38/32; 45/23; 45/29; 45/77; 631/158/855; 631/158/857; 631/326/2565/2134; 631/326/2565/547; Amino acids; Animals; Ants; Bacteria; Cephalotes; Digestive system; Essential nutrients; Herbivory; Humanities and Social Sciences; Intestinal microflora; Microbiomes; Microbiota; multidisciplinary; Mutualism; Nitrogen; Nutrients; Provisioning; Recycling; Science; Science (multidisciplinary); Symbionts; Symbiosis; Urea; Uric acid
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-018-03357-y

Nitrogen acquisition is a major challenge for herbivorous animals, and the repeated origins of herbivory across the ants have raised expectations that nutritional symbionts have shaped their diversification. Direct evidence for N provisioning by internally housed symbionts is rare in animals; among the ants, it has been documented for just one lineage. In this study we dissect functional contributions by bacteria from a conserved, multi-partite gut symbiosis in herbivorous Cephalotes ants through in vivo experiments, metagenomics, and in vitro assays. Gut bacteria recycle urea, and likely uric acid, using recycled N to synthesize essential amino acids that are acquired by hosts in substantial quantities. Specialized core symbionts of 17 studied Cephalotes species encode the pathways directing these activities, and several recycle N in vitro. These findings point to a highly efficient N economy, and a nutritional mutualism preserved for millions of years through the derived behaviors and gut anatomy of Cephalotes ants. Gut bacteria are prevalent across insects including ants, but their precise roles are often unclear. Here, Hu et al. show that microbes aid ants by recycling nitrogen into bio-available amino acids. This function is conserved across the turtle ants, suggesting an ancient nutritional mutualism.