QTL Mapping for Haploid Inducibility Using Genotyping by Sequencing in Maize

Doubled haploid (DH) technology in maize takes advantage of in vivo haploid induction (HI) triggered by pollination of donors of interest with inducer genotypes. However, the ability of different donors to be induced-inducibility (IND), varies among germplasm and the underlying molecular mechanisms...

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Bibliographic Details
Published in:Plants (Basel) 2022-03, Vol.11 (7), p.878
Main Authors: Trampe, Benjamin, Batîru, Grigorii, Pereira da Silva, Arthur, Frei, Ursula Karoline, Lübberstedt, Thomas
Format: Article
Language:English
Subjects:
Chromosomes
Corn
doubled haploids
Experiments
Gene mapping
Genomes
Genotype & phenotype
Genotypes
Genotyping
genotyping by sequencing
Germplasm
haploid inducibility
haploid kernels
Inbreeding
maize
Mapping
Molecular modelling
Phenotypic variations
Plant reproduction
Pollination
Population
QTL mapping
Quantitative trait loci
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Summary:Doubled haploid (DH) technology in maize takes advantage of in vivo haploid induction (HI) triggered by pollination of donors of interest with inducer genotypes. However, the ability of different donors to be induced-inducibility (IND), varies among germplasm and the underlying molecular mechanisms are still unclear. In this study, the phenotypic variation for IND in a mapping population of temperate inbred lines was evaluated to identify regions in the maize genome associated with IND. A total of 247 F families derived from a biparental cross of two elite inbred lines, A427 and CR1Ht, were grown in three different locations and Inclusive Composite Interval Mapping (ICIM) was used to identify quantitative trait loci (QTL) for IND. In total, four QTL were detected, explaining 37.4% of the phenotypic variance. No stable QTL was found across locations. The joint analysis revealed QTL × location interactions, suggesting minor QTL control IND, which are affected by the environment.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11070878