Asymmetric Mach–Zehnder Interferometric Biosensing for Quantitative and Sensitive Multiplex Detection of Anti-SARS-CoV-2 Antibodies in Human Plasma

The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic has once more emphasized the urgent need for accurate and fast point-of-care (POC) diagnostics for outbreak control and prevention. The main challenge in the development of POC in vitro diagnostics (IVD) is to combine a short...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-07, Vol.12 (8), p.553
Main Authors: Besselink, Geert, Schütz-Trilling, Anke, Veerbeek, Janneke, Verbruggen, Michelle, van der Meer, Adriaan, Schonenberg, Rens, Dam, Henk, Evers, Kevin, Lindhout, Ernst, Garritsen, Anja, van Amerongen, Aart, Knoben, Wout, Scheres, Luc
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Asymmetry
Biomarkers
biosensor
Biosensors
Blood plasma
Coronaviruses
COVID-19
diagnostics
Immune system
Integrated circuits
Interferometers
Light
Mach-Zehnder interferometers
Methods
Miniaturization
Molecular diagnostic techniques
Multiplexing
Nucleocapsids
Pandemics
Photonics
Proteins
SARS-CoV-2
Semiconductors
Sensitivity analysis
Sensors
Serology
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Sodium
Spike protein
Testing
Viral diseases
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Summary:The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic has once more emphasized the urgent need for accurate and fast point-of-care (POC) diagnostics for outbreak control and prevention. The main challenge in the development of POC in vitro diagnostics (IVD) is to combine a short time to result with a high sensitivity, and to keep the testing cost-effective. In this respect, sensors based on photonic integrated circuits (PICs) may offer advantages as they have features such as a high analytical sensitivity, capability for multiplexing, ease of miniaturization, and the potential for high-volume manufacturing. One special type of PIC sensor is the asymmetric Mach–Zehnder Interferometer (aMZI), which is characterized by a high and tunable analytical sensitivity. The current work describes the application of an aMZI-based biosensor platform for sensitive and multiplex detection of anti-SARS-CoV-2 antibodies in human plasma samples using the spike protein (SP), the receptor-binding domain (RBD), and the nucleocapsid protein (NP) as target antigens. The results are in good agreement with several CE-IVD marked reference methods and demonstrate the potential of the aMZI biosensor technology for further development into a photonic IVD platform.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12080553