Cold Atmospheric Plasma Promotes the Immunoreactivity of Granulocytes In Vitro

Cold atmospheric plasma (CAP) reduces bacteria and interacts with tissues and cells, thus improving wound healing. The CAP-related induction of neutrophils was recently described in stained sections of wound tissue in mice. Consequently, this study aimed to examine the functionality of human polymor...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2021-06, Vol.11 (6), p.902
Main Authors: Kupke, Laura S, Arndt, Stephanie, Lenzer, Simon, Metz, Sophia, Unger, Petra, Zimmermann, Julia L, Bosserhoff, Anja-Katrin, Gruber, Michael, Karrer, Sigrid
Format: Article
Language:English
Subjects:
Apoptosis
Apoptosis - drug effects
Cell adhesion & migration
cold atmospheric plasma
Cytokines
Experiments
Fibroblasts
Flow cytometry
Gene Expression Regulation - drug effects
Gene Expression Regulation - immunology
Granulocytes
Granulocytes - cytology
Granulocytes - immunology
Humans
Immunoreactivity
Integrins - immunology
Leukocyte migration
Leukocytes (granulocytic)
Leukocytes (neutrophilic)
Leukocytes (polymorphonuclear)
live-cell imaging
NETosis
Nitrogen dioxide
Plasma
Plasma Gases - pharmacology
Reactive nitrogen species
Reactive Nitrogen Species - immunology
Reactive oxygen species
Reactive Oxygen Species - immunology
Selectins - immunology
Software
Wound healing
wound treatment
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Summary:Cold atmospheric plasma (CAP) reduces bacteria and interacts with tissues and cells, thus improving wound healing. The CAP-related induction of neutrophils was recently described in stained sections of wound tissue in mice. Consequently, this study aimed to examine the functionality of human polymorphonuclear cells (PMN)/granulocytes through either a plasma-treated solution (PTS) or the direct CAP treatment with different plasma modes and treatment durations. PTS analysis yielded mode-dependent differences in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) after CAP treatment. Live-cell imaging did not show any chemo-attractive or NETosis-inducing effect on PMNs treated with PTS. The time to maximum ROS production (T ROS) in PMNs was reduced by PTS and direct CAP treatment. PMNs directly treated with CAP showed an altered cell migration dependent on the treatment duration as well as decreased T ROS without inducing apoptosis. Additionally, flow cytometry showed enhanced integrin and selectin expression, as a marker of activation, on PMN surfaces. In conclusion, the modification of PMN immunoreactivity may be a main supporting mechanism for CAP-induced improvement in wound healing.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom11060902