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Isolation, characterization, and pathogenicity assessment of Corynebacterium pseudotuberculosis biovar equi strains from alpacas ( Vicugna pacos ) in China
is a zoonotic pathogen that causes lymphadenitis in humans, livestock, and wildlife. In this study, biovar equi strains were isolated from three alpacas. Antibiotic susceptibility tests and pathogenicity tests were also conducted. Moreover, one strain was sequenced using DNBSEQ and Oxford Nanopore t...
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Published in: | Frontiers in microbiology 2023-07, Vol.14, p.1206187-1206187 |
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Main Authors: | , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | is a zoonotic pathogen that causes lymphadenitis in humans, livestock, and wildlife. In this study,
biovar equi strains were isolated from three alpacas. Antibiotic susceptibility tests and pathogenicity tests were also conducted. Moreover, one strain was sequenced using DNBSEQ and Oxford Nanopore technology. The three strains exhibited resistance to aztreonam, fosfomycin, and nitrofurantoin. The median lethal doses (LD
) of strains G1, S2 and BA3 in experimentally infected mice was 1.66 × 10
CFU, 3.78 × 10
CFU and 3.78 × 10
CFU, respectively. The sequencing of strain G1 resulted in the assembly of a chromosomal scaffold comprising 2,379,166 bp with a G + C content of 52.06%. Genome analysis of strain G1 revealed the presence of 48 virulence genes and 5 antibiotic resistance genes (ARGs). Comparative genomic analysis demonstrates a high degree of genetic similarity among
strains, in contrast to other
species, with a clear delineation between strains belonging to the two biovars (ovis and equi). The data of the present study contribute to a better understanding of the properties of
biovar equi strains and the potential risk they pose to alpacas and other livestock, as well as the necessity of ongoing surveillance and monitoring of infectious diseases in animals. |
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ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2023.1206187 |