Loading…

Isolation, characterization, and pathogenicity assessment of Corynebacterium pseudotuberculosis biovar equi strains from alpacas ( Vicugna pacos ) in China

is a zoonotic pathogen that causes lymphadenitis in humans, livestock, and wildlife. In this study, biovar equi strains were isolated from three alpacas. Antibiotic susceptibility tests and pathogenicity tests were also conducted. Moreover, one strain was sequenced using DNBSEQ and Oxford Nanopore t...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in microbiology 2023-07, Vol.14, p.1206187-1206187
Main Authors: Meng, Wanyu, Chen, Shanyu, Huang, Lin, Yang, Jinpeng, Zhang, Wenqing, Zhong, Zhijun, Zhou, Ziyao, Liu, Haifeng, Fu, Hualin, He, Tingmei, Peng, Guangneng
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:is a zoonotic pathogen that causes lymphadenitis in humans, livestock, and wildlife. In this study, biovar equi strains were isolated from three alpacas. Antibiotic susceptibility tests and pathogenicity tests were also conducted. Moreover, one strain was sequenced using DNBSEQ and Oxford Nanopore technology. The three strains exhibited resistance to aztreonam, fosfomycin, and nitrofurantoin. The median lethal doses (LD ) of strains G1, S2 and BA3 in experimentally infected mice was 1.66 × 10 CFU, 3.78 × 10 CFU and 3.78 × 10 CFU, respectively. The sequencing of strain G1 resulted in the assembly of a chromosomal scaffold comprising 2,379,166 bp with a G + C content of 52.06%. Genome analysis of strain G1 revealed the presence of 48 virulence genes and 5 antibiotic resistance genes (ARGs). Comparative genomic analysis demonstrates a high degree of genetic similarity among strains, in contrast to other species, with a clear delineation between strains belonging to the two biovars (ovis and equi). The data of the present study contribute to a better understanding of the properties of biovar equi strains and the potential risk they pose to alpacas and other livestock, as well as the necessity of ongoing surveillance and monitoring of infectious diseases in animals.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2023.1206187