AC amplification gain in organic electrochemical transistors for impedance-based single cell sensors

Research on electrolyte-gated and organic electrochemical transistor (OECT) architectures is motivated by the prospect of a highly biocompatible interface capable of amplifying bioelectronic signals at the site of detection. Despite many demonstrations in these directions, a quantitative model for O...

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Bibliographic Details
Published in:Nature communications 2022-09, Vol.13 (1), p.5423-5423, Article 5423
Main Authors: Bonafè, Filippo, Decataldo, Francesco, Zironi, Isabella, Remondini, Daniel, Cramer, Tobias, Fraboni, Beatrice
Format: Article
Language:English
Subjects:
13/56
142/126
147/3
639/166/985
639/301/1005/1009
639/638/440/94
9/10
Adhesion
Amplification
Biocompatibility
Bioelectricity
Biosensors
Cell adhesion
Cell adhesion & migration
Electrochemistry
Experiments
Glioblastoma
Humanities and Social Sciences
Impedance
Mathematical models
Microelectrodes
Microparticles
multidisciplinary
Optimization
Science
Science (multidisciplinary)
Semiconductor devices
Sensors
Transistors
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Summary:Research on electrolyte-gated and organic electrochemical transistor (OECT) architectures is motivated by the prospect of a highly biocompatible interface capable of amplifying bioelectronic signals at the site of detection. Despite many demonstrations in these directions, a quantitative model for OECTs as impedance biosensors is still lacking. We overcome this issue by introducing a model experiment where we simulate the detection of a single cell by the impedance sensing of a dielectric microparticle. The highly reproducible experiment allows us to study the impact of transistor geometry and operation conditions on device sensitivity. With the data we rationalize a mathematical model that provides clear guidelines for the optimization of OECTs as single cell sensors, and we verify the quantitative predictions in an in-vitro experiment. In the optimized geometry, the OECT-based impedance sensor allows to record single cell adhesion and detachment transients, showing a maximum gain of 20.2±0.9 dB with respect to a single electrode-based impedance sensor. The authors develop a quantitative description of alternating current amplification gain in organic electrochemical transistors. The findings are applied to achieve detection of single glioblastoma cell adhesion with 20 dB gain compared to microelectrodes.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-022-33094-2