Biodegradable Polyesters and Low Molecular Weight Polyethylene in Soil: Interrelations of Material Properties, Soil Organic Matter Substances, and Microbial Community

• Published in: International journal of molecular sciences 2022-12, Vol.23 (24), p.15976
• Main Authors: Šerá, Jana, Huynh, Florence, Ly, Faith, Vinter, Štěpán, Kadlečková, Markéta, Krátká, Vendula, Máčalová, Daniela, Koutný, Marek, Wallis, Christopher
• Format: Article
• Language: English
• Subjects: Bacteria; Bacteria - genetics; Biodegradability; Biodegradable materials; Biodegradation; Biofilms; Biomass; Carbon; Consortia; Enzymes; Experiments; Fluorescence; Fungi; Humic substances; Low molecular weights; Material properties; Metagenomics; Microbial Consortia; Microorganisms; Microscopy; Mineralization; Molecular Weight; Morphology; Organic matter; Organic soils; Plastic pollution; Plastics; Polyester resins; Polyesters; Polyethylene; Polyethylenes; Polyhydroxybutyrate; Polymers; Soil; Soil investigations; Soil Microbiology; Soil microorganisms; soil organic matter; Soil properties; Waxes
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232415976

Conventional and also biodegradable polymer microplastics have started to be broadly present in the environment, if they end up in soil, they may influence both abiotic and biotic soil properties. In this study, the interactions of polyethylene wax together with three biodegradable polyesters PLA, PHB and PBAT with a soil matrix were investigated over a 1-year incubation period. Soil organic matter content was measured using UV-VIS, the microbial biomass amount was measured using qPCR, the mineralisation of polymers was measured using UGA 3000, the surface of polymers was observed with SEM, live/dead microorganisms were determined by fluorescent microscopy and microbial consortia diversity was analyzed using NGS. The amount of humic substances was generally higher in incubations with slowly degrading polyesters, but the effect was temporary. The microbial biomass grew during the incubations; the addition of PHB enhanced fungal biomass whereas PE wax enhanced bacterial biomass. Fungal microbial consortia diversity was altered in incubations with PHB and PBAT. Interestingly, these two polyesters were also covered in biofilm, probably fungal. No such trend was observed in a metagenomic analysis of bacteria, although, bacterial biofilm was probably formed on the PE520 surface. Different methods confirmed the effect of certain polymers on the soil environment.