Blood Metabolite Profiling of Antarctic Expedition Members: An 1H NMR Spectroscopy-Based Study

Serum samples from eight participants during the XV winter-over at Concordia base (Antarctic expedition) collected at defined time points, including predeparture, constituted the key substrates for a specific metabolomics study. To ascertain acute changes and chronic adaptation to hypoxia, the metab...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-05, Vol.24 (9), p.8459
Main Authors: Del Coco, Laura, Greco, Marco, Inguscio, Alessandra, Munir, Anas, Danieli, Antonio, Cossa, Luca, Musarò, Debora, Coscia, Maria Rosaria, Fanizzi, Francesco Paolo, Maffia, Michele
Format: Article
Language:English
Subjects:
Adaptation
Altitude
Antarctica
Atmospheric pressure
Chronic illnesses
Concordia base
Glutamine
Glycoproteins
Hypoxia
Inflammation
Lipids
Magnetic resonance spectroscopy
Metabolism
Metabolites
Metabolomics
Multivariate analysis
NMR
NMR spectroscopy
Nuclear magnetic resonance
Physiology
Principal components analysis
Spectroscopic analysis
Spectrum analysis
winter-over
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Summary:Serum samples from eight participants during the XV winter-over at Concordia base (Antarctic expedition) collected at defined time points, including predeparture, constituted the key substrates for a specific metabolomics study. To ascertain acute changes and chronic adaptation to hypoxia, the metabolic profiles of the serum samples were analyzed using NMR spectroscopy, with principal components analysis (PCA) followed by partial least squares and orthogonal partial least squares discriminant analyses (PLS-DA and OPLS-DA) used as supervised classification methods. Multivariate data analyses clearly highlighted an adaptation period characterized by an increase in the levels of circulating glutamine and lipids, mobilized to supply the body energy needs. At the same time, a reduction in the circulating levels of glutamate and N-acetyl glycoproteins, stress condition indicators, and proinflammatory markers were also found in the NMR data investigation. Subsequent pathway analysis showed possible perturbations in metabolic processes, potentially related to the physiological adaptation, predominantly found by comparing the baseline (at sea level, before mission onset), the base arrival, and the mission ending collected values.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24098459