Molecular epidemiology and clinical characteristics of hepatitis delta virus (HDV) infected patients with elevated transaminases in Shanghai, China

This retrospective study involved 225 serum samples from HBsAg positive hospitalized patients from October 2010 to April 2013. HDV-specific RT-nested PCR was used to amplify HDV RNA. HDV genotypes were characterized by Next-generation sequencing (NGS), followed by phylogenetic analyses. HDV/HBV co-i...

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Bibliographic Details
Published in:BMC infectious diseases 2020-08, Vol.20 (1), p.1-565, Article 565
Main Authors: Wu, Shanshan, Zhang, Yi, Tang, Yuyan, Yao, Ting, Lv, Mengjiao, Tang, Zhenghao, Zang, Guoqing, Yu, Yongsheng, Chen, Xiaohua
Format: Article
Language:English
Subjects:
Cirrhosis
Cloning
Comorbidity
Comparative analysis
Deoxyribonucleic acid
DNA
Enzymes
Epidemiology
Gene amplification
Genetic aspects
Genetic diversity
Genomes
Genomics
Genotype & phenotype
Genotype distribution
Genotypes
Health aspects
Hepatitis
Hepatitis B
Hepatitis B surface antigen
Hepatitis Delta virus
Infections
Laboratories
Liver cancer
Liver cirrhosis
Next-generation sequencing
Phylogenetics
Phylogeny
Polymerase chain reaction
Ribonucleic acid
RNA
RT-nested PCR
Transaminases
Viruses
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Summary:This retrospective study involved 225 serum samples from HBsAg positive hospitalized patients from October 2010 to April 2013. HDV-specific RT-nested PCR was used to amplify HDV RNA. HDV genotypes were characterized by Next-generation sequencing (NGS), followed by phylogenetic analyses. HDV/HBV co-infected patients and HBV mono-infected patients were compared clinically and virologically. Out of the 225 HBsAg-positive serum samples with elevated transaminases, HDV-RNA was identified in 11 (4.9%) patients. The HBV loads in the HDV positive group were significantly lower than the HDV negative HBV-infected patients. The aminotransferase enzymes were significantly higher in HDV/HBV co-infected compared to HDV negative patients (P < 0.05). Phylogenetic analyses indicated that HDV-2 genotype being the predominant genotype, other HDV genotypes were not observed. HDV/HBV patients were significantly associated with a rather unfavourable clinical outcome. In summary, the prevalence of HDV infection in patients with elevated transaminases is not low and the predominance of HDV genotype 2 infection in Shanghai. This finding helps us to better understand the correlation of HDV/HBV co-infection. Moreover, Next-generation sequencing (NGS) technologies provide a rapid, precise method for generating HDV genomes to define infecting genotypes.
ISSN:1471-2334
1471-2334
DOI:10.1186/s12879-020-05275-1