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Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production
(formerly known as ) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharmaceuticals. During the last decades, relevant...
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| Published in: | Metabolites 2022-04, Vol.12 (4), p.346 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c528t-5ce0ba6dc49a2edb5ef5d1b8e9e713fefa0b619fa97d89870589fe1be230243a3 |
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| container_issue | 4 |
| container_start_page | 346 |
| container_title | Metabolites |
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| creator | Bustos, Cristina Quezada, Johan Veas, Rhonda Altamirano, Claudia Braun-Galleani, Stephanie Fickers, Patrick Berrios, Julio |
| description | (formerly known as
) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharmaceuticals. During the last decades, relevant bioprocess progress has been achieved in order to increase recombinant protein productivity and to reduce production costs. More recently, the improvement of cell features and performance has also been considered for this aim, and promising strategies with a direct and substantial impact on protein productivity have been reported. In this review, cell engineering approaches including metabolic engineering and energy supply, transcription factor modulation, and manipulation of routes involved in folding and secretion of recombinant protein are discussed. A lack of studies performed at the higher-scale bioreactor involving optimisation of cultivation parameters is also evidenced, which highlights new research aims to be considered. |
| doi_str_mv | 10.3390/metabo12040346 |
| format | article |
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) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharmaceuticals. During the last decades, relevant bioprocess progress has been achieved in order to increase recombinant protein productivity and to reduce production costs. More recently, the improvement of cell features and performance has also been considered for this aim, and promising strategies with a direct and substantial impact on protein productivity have been reported. In this review, cell engineering approaches including metabolic engineering and energy supply, transcription factor modulation, and manipulation of routes involved in folding and secretion of recombinant protein are discussed. A lack of studies performed at the higher-scale bioreactor involving optimisation of cultivation parameters is also evidenced, which highlights new research aims to be considered.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>35448535</pmid><doi>10.3390/metabo12040346</doi><orcidid>https://orcid.org/0000-0002-2600-5833</orcidid><orcidid>https://orcid.org/0000-0001-9503-2993</orcidid><orcidid>https://orcid.org/0000-0001-5777-0709</orcidid><orcidid>https://orcid.org/0000-0003-1318-7575</orcidid><orcidid>https://orcid.org/0000-0002-6180-0023</orcidid><orcidid>https://orcid.org/0000-0001-8901-9135</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Metabolites, 2022-04, Vol.12 (4), p.346 |
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| language | eng |
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| subjects | Alcohol Biochemistry Biomass Bioreactors Biotechnologie Biotechnology Carbon cell engineering Dehydrogenases Endocrinology, Diabetes and Metabolism Gene expression Genetic engineering Glycerol Komagataella phaffii Life sciences Metabolic engineering Metabolism Metabolites Molecular Biology Oxidative stress Pichia pastoris Productivity Protein folding Proteins recombinant protein Review Sciences du vivant |
| title | Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production |
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