Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease

Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alo...

Full description

Saved in:
Bibliographic Details
Published in:International journal of nanomedicine 2016-01, Vol.11 (Issue 1), p.259-267
Main Authors: Firouzamandi, Masoumeh, Moeini, Hassan, Hosseini, Seyed Davood, Bejo, Mohd Hair, Omar, Abdul Rahman, Mehrbod, Parvaneh, El Zowalaty, Mohamed E, Webster, Thomas J, Ideris, Aini
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - immunology
Chick Embryo
Chickens
Deoxyribonucleic acid
Dextran-spermine nanoparticles
Dextrans
Dextrans - chemistry
DNA
DNA vaccine
Embryos
Female
Genes
Hemagglutination Inhibition Tests
Hemagglutinins - administration & dosage
Hemagglutinins - genetics
Hemagglutinins - metabolism
in ovo
Lectins
Medical research
Microscopy
Nanoparticles
Nanoparticles - chemistry
Nanotechnology
Newcastle disease
Newcastle Disease - genetics
Newcastle Disease - immunology
Newcastle Disease - prevention & control
Newcastle disease virus
Newcastle disease virus - immunology
Original Research
Ovum - immunology
Paramyxoviridae
Particle size
Polyamines
Spermine - chemistry
Sunscreening agents
Vaccination
Vaccines
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
Veterinary medicine
Viral Vaccines - administration & dosage
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c572t-440a0db07a7915492c2b5c59579183b605a392c7ff99c24ee9e368743d8188643
cites
container_end_page 267
container_issue Issue 1
container_start_page 259
container_title International journal of nanomedicine
container_volume 11
creator Firouzamandi, Masoumeh
Moeini, Hassan
Hosseini, Seyed Davood
Bejo, Mohd Hair
Omar, Abdul Rahman
Mehrbod, Parvaneh
El Zowalaty, Mohamed E
Webster, Thomas J
Ideris, Aini
description Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alone or as a mixture of the pDNA and dextran-spermine (D-SPM), a nanoparticle used for pDNA delivery. The results showed that in ovo vaccination with 40 μg pDNA/egg alone induced high levels of antibody titer (P0.05). Higher antibody titer was observed in the group immunized with 40 μg pDNA/egg at 4 weeks postvaccination. The findings also showed that vaccination with 40 μg pDNA/egg alone was able to confer protection against Newcastle disease virus strain NDIBS002 in two out of seven SPF chickens. Although the chickens produced antibody titers 3 weeks after in ovo vaccination, it was not sufficient to provide complete protection to the chickens from lethal viral challenge. In addition, vaccination with pDNA/D-SPM complex did not induce high antibody titer when compared with naked pDNA. Therefore, it was concluded that DNA vaccination with plasmid internal ribosome entry site-HN/F can be suitable for in ovo application against ND, whereas D-SPM is not recommended for in ovo gene delivery.
doi_str_mv 10.2147/IJN.S92225
format article
fullrecord <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_e1115e6b36cf4fd2ad21007a877a9206</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A506650634</galeid><doaj_id>oai_doaj_org_article_e1115e6b36cf4fd2ad21007a877a9206</doaj_id><sourcerecordid>A506650634</sourcerecordid><originalsourceid>FETCH-LOGICAL-c572t-440a0db07a7915492c2b5c59579183b605a392c7ff99c24ee9e368743d8188643</originalsourceid><addsrcrecordid>eNptkl2LEzEUhgdR3HX1xh8gAW9EbM13Zm6Esn5Vliqo1yHNnJmmzCQ1mamrv8kfaWZb112REJK8ec9zOIdTFI8JnlPC1cvlh9X8c0UpFXeKU0JUOaOYsLs37ifFg5S2GAtVyup-cUJlyThX-LT49SnCzkQzuOBfILvJVztAdD-PivE1ch6FfUB7Y63zVzoKDarhcojGz9IOYu88IG98yKjB2Q7Q69XiGADIBrjcRUjJ-RYNG0DNmCbIxN5Ab9q2Gwfnc5oWPCRkWuN8GtAKvluThkyrXQKT4GFxrzFdgkfH86z4-vbNl_P3s4uP75bni4uZFYoOM86xwfUaK6MqInhFLV0LKyqRnyVbSywMy6JqmqqylANUwGSpOKtLUpaSs7NieeDWwWz1LrrexB86GKevhBBbfaxTAyFEgFwzaRve1NTUlOCcuVTKVBTLzHp1YO3GdQ-1BZ-71t2C3v7xbqPbsNdcEak4zYBnR0AM30ZIg-5dstB1xkMYkyZKiqpiik7Wp_9Yt2GMPrdK59-SUK6Y-utqTS7A-SbkvHaC6oXAUubNph7M_-PKq4be2eChcVm_FfD8EGBjSClCc10jwXqaU53nVB_mNJuf3OzKtfXPYLLfLenkPw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2238124737</pqid></control><display><type>article</type><title>Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease</title><source>Taylor &amp; Francis_OA刊</source><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Firouzamandi, Masoumeh ; Moeini, Hassan ; Hosseini, Seyed Davood ; Bejo, Mohd Hair ; Omar, Abdul Rahman ; Mehrbod, Parvaneh ; El Zowalaty, Mohamed E ; Webster, Thomas J ; Ideris, Aini</creator><creatorcontrib>Firouzamandi, Masoumeh ; Moeini, Hassan ; Hosseini, Seyed Davood ; Bejo, Mohd Hair ; Omar, Abdul Rahman ; Mehrbod, Parvaneh ; El Zowalaty, Mohamed E ; Webster, Thomas J ; Ideris, Aini</creatorcontrib><description>Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alone or as a mixture of the pDNA and dextran-spermine (D-SPM), a nanoparticle used for pDNA delivery. The results showed that in ovo vaccination with 40 μg pDNA/egg alone induced high levels of antibody titer (P&lt;0.05) in specific pathogen-free (SPF) chickens at 3 and 4 weeks postvaccination compared to 2 weeks postvaccination. Hemagglutination inhibition (HI) titer was not significantly different between groups injected with 40 μg pDNA + 64 μg D-SPM and 40 μg pDNA at 4 weeks postvaccination (P&gt;0.05). Higher antibody titer was observed in the group immunized with 40 μg pDNA/egg at 4 weeks postvaccination. The findings also showed that vaccination with 40 μg pDNA/egg alone was able to confer protection against Newcastle disease virus strain NDIBS002 in two out of seven SPF chickens. Although the chickens produced antibody titers 3 weeks after in ovo vaccination, it was not sufficient to provide complete protection to the chickens from lethal viral challenge. In addition, vaccination with pDNA/D-SPM complex did not induce high antibody titer when compared with naked pDNA. Therefore, it was concluded that DNA vaccination with plasmid internal ribosome entry site-HN/F can be suitable for in ovo application against ND, whereas D-SPM is not recommended for in ovo gene delivery.</description><identifier>ISSN: 1178-2013</identifier><identifier>ISSN: 1176-9114</identifier><identifier>EISSN: 1178-2013</identifier><identifier>DOI: 10.2147/IJN.S92225</identifier><identifier>PMID: 26834470</identifier><language>eng</language><publisher>New Zealand: Dove Medical Press Limited</publisher><subject>Animals ; Antibodies, Viral - immunology ; Chick Embryo ; Chickens ; Deoxyribonucleic acid ; Dextran-spermine nanoparticles ; Dextrans ; Dextrans - chemistry ; DNA ; DNA vaccine ; Embryos ; Female ; Genes ; Hemagglutination Inhibition Tests ; Hemagglutinins - administration &amp; dosage ; Hemagglutinins - genetics ; Hemagglutinins - metabolism ; in ovo ; Lectins ; Medical research ; Microscopy ; Nanoparticles ; Nanoparticles - chemistry ; Nanotechnology ; Newcastle disease ; Newcastle Disease - genetics ; Newcastle Disease - immunology ; Newcastle Disease - prevention &amp; control ; Newcastle disease virus ; Newcastle disease virus - immunology ; Original Research ; Ovum - immunology ; Paramyxoviridae ; Particle size ; Polyamines ; Spermine - chemistry ; Sunscreening agents ; Vaccination ; Vaccines ; Vaccines, DNA - administration &amp; dosage ; Vaccines, DNA - immunology ; Veterinary medicine ; Viral Vaccines - administration &amp; dosage</subject><ispartof>International journal of nanomedicine, 2016-01, Vol.11 (Issue 1), p.259-267</ispartof><rights>COPYRIGHT 2016 Dove Medical Press Limited</rights><rights>2016. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Firouzamandi et al. This work is published and licensed by Dove Medical Press Limited 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c572t-440a0db07a7915492c2b5c59579183b605a392c7ff99c24ee9e368743d8188643</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2238124737/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2238124737?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25751,27922,27923,37010,37011,44588,53789,53791,74896</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26834470$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Firouzamandi, Masoumeh</creatorcontrib><creatorcontrib>Moeini, Hassan</creatorcontrib><creatorcontrib>Hosseini, Seyed Davood</creatorcontrib><creatorcontrib>Bejo, Mohd Hair</creatorcontrib><creatorcontrib>Omar, Abdul Rahman</creatorcontrib><creatorcontrib>Mehrbod, Parvaneh</creatorcontrib><creatorcontrib>El Zowalaty, Mohamed E</creatorcontrib><creatorcontrib>Webster, Thomas J</creatorcontrib><creatorcontrib>Ideris, Aini</creatorcontrib><title>Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease</title><title>International journal of nanomedicine</title><addtitle>Int J Nanomedicine</addtitle><description>Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alone or as a mixture of the pDNA and dextran-spermine (D-SPM), a nanoparticle used for pDNA delivery. The results showed that in ovo vaccination with 40 μg pDNA/egg alone induced high levels of antibody titer (P&lt;0.05) in specific pathogen-free (SPF) chickens at 3 and 4 weeks postvaccination compared to 2 weeks postvaccination. Hemagglutination inhibition (HI) titer was not significantly different between groups injected with 40 μg pDNA + 64 μg D-SPM and 40 μg pDNA at 4 weeks postvaccination (P&gt;0.05). Higher antibody titer was observed in the group immunized with 40 μg pDNA/egg at 4 weeks postvaccination. The findings also showed that vaccination with 40 μg pDNA/egg alone was able to confer protection against Newcastle disease virus strain NDIBS002 in two out of seven SPF chickens. Although the chickens produced antibody titers 3 weeks after in ovo vaccination, it was not sufficient to provide complete protection to the chickens from lethal viral challenge. In addition, vaccination with pDNA/D-SPM complex did not induce high antibody titer when compared with naked pDNA. Therefore, it was concluded that DNA vaccination with plasmid internal ribosome entry site-HN/F can be suitable for in ovo application against ND, whereas D-SPM is not recommended for in ovo gene delivery.</description><subject>Animals</subject><subject>Antibodies, Viral - immunology</subject><subject>Chick Embryo</subject><subject>Chickens</subject><subject>Deoxyribonucleic acid</subject><subject>Dextran-spermine nanoparticles</subject><subject>Dextrans</subject><subject>Dextrans - chemistry</subject><subject>DNA</subject><subject>DNA vaccine</subject><subject>Embryos</subject><subject>Female</subject><subject>Genes</subject><subject>Hemagglutination Inhibition Tests</subject><subject>Hemagglutinins - administration &amp; dosage</subject><subject>Hemagglutinins - genetics</subject><subject>Hemagglutinins - metabolism</subject><subject>in ovo</subject><subject>Lectins</subject><subject>Medical research</subject><subject>Microscopy</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Nanotechnology</subject><subject>Newcastle disease</subject><subject>Newcastle Disease - genetics</subject><subject>Newcastle Disease - immunology</subject><subject>Newcastle Disease - prevention &amp; control</subject><subject>Newcastle disease virus</subject><subject>Newcastle disease virus - immunology</subject><subject>Original Research</subject><subject>Ovum - immunology</subject><subject>Paramyxoviridae</subject><subject>Particle size</subject><subject>Polyamines</subject><subject>Spermine - chemistry</subject><subject>Sunscreening agents</subject><subject>Vaccination</subject><subject>Vaccines</subject><subject>Vaccines, DNA - administration &amp; dosage</subject><subject>Vaccines, DNA - immunology</subject><subject>Veterinary medicine</subject><subject>Viral Vaccines - administration &amp; dosage</subject><issn>1178-2013</issn><issn>1176-9114</issn><issn>1178-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNptkl2LEzEUhgdR3HX1xh8gAW9EbM13Zm6Esn5Vliqo1yHNnJmmzCQ1mamrv8kfaWZb112REJK8ec9zOIdTFI8JnlPC1cvlh9X8c0UpFXeKU0JUOaOYsLs37ifFg5S2GAtVyup-cUJlyThX-LT49SnCzkQzuOBfILvJVztAdD-PivE1ch6FfUB7Y63zVzoKDarhcojGz9IOYu88IG98yKjB2Q7Q69XiGADIBrjcRUjJ-RYNG0DNmCbIxN5Ab9q2Gwfnc5oWPCRkWuN8GtAKvluThkyrXQKT4GFxrzFdgkfH86z4-vbNl_P3s4uP75bni4uZFYoOM86xwfUaK6MqInhFLV0LKyqRnyVbSywMy6JqmqqylANUwGSpOKtLUpaSs7NieeDWwWz1LrrexB86GKevhBBbfaxTAyFEgFwzaRve1NTUlOCcuVTKVBTLzHp1YO3GdQ-1BZ-71t2C3v7xbqPbsNdcEak4zYBnR0AM30ZIg-5dstB1xkMYkyZKiqpiik7Wp_9Yt2GMPrdK59-SUK6Y-utqTS7A-SbkvHaC6oXAUubNph7M_-PKq4be2eChcVm_FfD8EGBjSClCc10jwXqaU53nVB_mNJuf3OzKtfXPYLLfLenkPw</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Firouzamandi, Masoumeh</creator><creator>Moeini, Hassan</creator><creator>Hosseini, Seyed Davood</creator><creator>Bejo, Mohd Hair</creator><creator>Omar, Abdul Rahman</creator><creator>Mehrbod, Parvaneh</creator><creator>El Zowalaty, Mohamed E</creator><creator>Webster, Thomas J</creator><creator>Ideris, Aini</creator><general>Dove Medical Press Limited</general><general>Taylor &amp; Francis Ltd</general><general>Dove Medical Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QO</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20160101</creationdate><title>Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease</title><author>Firouzamandi, Masoumeh ; Moeini, Hassan ; Hosseini, Seyed Davood ; Bejo, Mohd Hair ; Omar, Abdul Rahman ; Mehrbod, Parvaneh ; El Zowalaty, Mohamed E ; Webster, Thomas J ; Ideris, Aini</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c572t-440a0db07a7915492c2b5c59579183b605a392c7ff99c24ee9e368743d8188643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Antibodies, Viral - immunology</topic><topic>Chick Embryo</topic><topic>Chickens</topic><topic>Deoxyribonucleic acid</topic><topic>Dextran-spermine nanoparticles</topic><topic>Dextrans</topic><topic>Dextrans - chemistry</topic><topic>DNA</topic><topic>DNA vaccine</topic><topic>Embryos</topic><topic>Female</topic><topic>Genes</topic><topic>Hemagglutination Inhibition Tests</topic><topic>Hemagglutinins - administration &amp; dosage</topic><topic>Hemagglutinins - genetics</topic><topic>Hemagglutinins - metabolism</topic><topic>in ovo</topic><topic>Lectins</topic><topic>Medical research</topic><topic>Microscopy</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Nanotechnology</topic><topic>Newcastle disease</topic><topic>Newcastle Disease - genetics</topic><topic>Newcastle Disease - immunology</topic><topic>Newcastle Disease - prevention &amp; control</topic><topic>Newcastle disease virus</topic><topic>Newcastle disease virus - immunology</topic><topic>Original Research</topic><topic>Ovum - immunology</topic><topic>Paramyxoviridae</topic><topic>Particle size</topic><topic>Polyamines</topic><topic>Spermine - chemistry</topic><topic>Sunscreening agents</topic><topic>Vaccination</topic><topic>Vaccines</topic><topic>Vaccines, DNA - administration &amp; dosage</topic><topic>Vaccines, DNA - immunology</topic><topic>Veterinary medicine</topic><topic>Viral Vaccines - administration &amp; dosage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Firouzamandi, Masoumeh</creatorcontrib><creatorcontrib>Moeini, Hassan</creatorcontrib><creatorcontrib>Hosseini, Seyed Davood</creatorcontrib><creatorcontrib>Bejo, Mohd Hair</creatorcontrib><creatorcontrib>Omar, Abdul Rahman</creatorcontrib><creatorcontrib>Mehrbod, Parvaneh</creatorcontrib><creatorcontrib>El Zowalaty, Mohamed E</creatorcontrib><creatorcontrib>Webster, Thomas J</creatorcontrib><creatorcontrib>Ideris, Aini</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>ProQuest research library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of nanomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Firouzamandi, Masoumeh</au><au>Moeini, Hassan</au><au>Hosseini, Seyed Davood</au><au>Bejo, Mohd Hair</au><au>Omar, Abdul Rahman</au><au>Mehrbod, Parvaneh</au><au>El Zowalaty, Mohamed E</au><au>Webster, Thomas J</au><au>Ideris, Aini</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease</atitle><jtitle>International journal of nanomedicine</jtitle><addtitle>Int J Nanomedicine</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>11</volume><issue>Issue 1</issue><spage>259</spage><epage>267</epage><pages>259-267</pages><issn>1178-2013</issn><issn>1176-9114</issn><eissn>1178-2013</eissn><abstract>Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alone or as a mixture of the pDNA and dextran-spermine (D-SPM), a nanoparticle used for pDNA delivery. The results showed that in ovo vaccination with 40 μg pDNA/egg alone induced high levels of antibody titer (P&lt;0.05) in specific pathogen-free (SPF) chickens at 3 and 4 weeks postvaccination compared to 2 weeks postvaccination. Hemagglutination inhibition (HI) titer was not significantly different between groups injected with 40 μg pDNA + 64 μg D-SPM and 40 μg pDNA at 4 weeks postvaccination (P&gt;0.05). Higher antibody titer was observed in the group immunized with 40 μg pDNA/egg at 4 weeks postvaccination. The findings also showed that vaccination with 40 μg pDNA/egg alone was able to confer protection against Newcastle disease virus strain NDIBS002 in two out of seven SPF chickens. Although the chickens produced antibody titers 3 weeks after in ovo vaccination, it was not sufficient to provide complete protection to the chickens from lethal viral challenge. In addition, vaccination with pDNA/D-SPM complex did not induce high antibody titer when compared with naked pDNA. Therefore, it was concluded that DNA vaccination with plasmid internal ribosome entry site-HN/F can be suitable for in ovo application against ND, whereas D-SPM is not recommended for in ovo gene delivery.</abstract><cop>New Zealand</cop><pub>Dove Medical Press Limited</pub><pmid>26834470</pmid><doi>10.2147/IJN.S92225</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1178-2013
ispartof International journal of nanomedicine, 2016-01, Vol.11 (Issue 1), p.259-267
issn 1178-2013
1176-9114
1178-2013
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_e1115e6b36cf4fd2ad21007a877a9206
source Taylor & Francis_OA刊; Publicly Available Content Database; PubMed Central
subjects Animals
Antibodies, Viral - immunology
Chick Embryo
Chickens
Deoxyribonucleic acid
Dextran-spermine nanoparticles
Dextrans
Dextrans - chemistry
DNA
DNA vaccine
Embryos
Female
Genes
Hemagglutination Inhibition Tests
Hemagglutinins - administration & dosage
Hemagglutinins - genetics
Hemagglutinins - metabolism
in ovo
Lectins
Medical research
Microscopy
Nanoparticles
Nanoparticles - chemistry
Nanotechnology
Newcastle disease
Newcastle Disease - genetics
Newcastle Disease - immunology
Newcastle Disease - prevention & control
Newcastle disease virus
Newcastle disease virus - immunology
Original Research
Ovum - immunology
Paramyxoviridae
Particle size
Polyamines
Spermine - chemistry
Sunscreening agents
Vaccination
Vaccines
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
Veterinary medicine
Viral Vaccines - administration & dosage
title Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T15%3A00%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation,%20characterization,%20and%20in%20ovo%20vaccination%20of%20dextran-spermine%20nanoparticle%20DNA%20vaccine%20coexpressing%20the%20fusion%20and%20hemagglutinin%20genes%20against%20Newcastle%20disease&rft.jtitle=International%20journal%20of%20nanomedicine&rft.au=Firouzamandi,%20Masoumeh&rft.date=2016-01-01&rft.volume=11&rft.issue=Issue%201&rft.spage=259&rft.epage=267&rft.pages=259-267&rft.issn=1178-2013&rft.eissn=1178-2013&rft_id=info:doi/10.2147/IJN.S92225&rft_dat=%3Cgale_doaj_%3EA506650634%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c572t-440a0db07a7915492c2b5c59579183b605a392c7ff99c24ee9e368743d8188643%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2238124737&rft_id=info:pmid/26834470&rft_galeid=A506650634&rfr_iscdi=true