The novel developed microsatellite markers revealed potential hybridization among Cymbidium species and the interspecies sub-division of C. goeringii and C. ensifolium

Orchids (Cymbidium spp.) exhibit significant variations in floral morphology, pollinator relations, and ecological habitats. Due to their exceptional economic and ornamental value, Cymbidium spp. have been commercially cultivated for centuries. SSR markers are extensively used genetic tools for biol...

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Published in:BMC plant biology 2023-10, Vol.23 (1), p.1-492, Article 492
Main Authors: Ning, Hui-Juan, Gui, Fang-Fang, Tian, En-Wei, Yang, Li-Yuan
Format: Article
Language:English
Subjects:
Annotations
Clustering
Cultivars
Cymbidium
Cymbidium goeringii
Gene flow
Genes
Genetic aspects
Genetic diversity
Genetic markers
Genetic research
Genetics
Hybridization
Inter-species sub-division
Microsatellites
Microsatellites (Genetics)
Neutrality
Orchids
Phylogenetics
Population genetics
Ribonucleic acid
RNA
RNA sequencing
RNA-Seq
Species
SSR-Marker
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Summary:Orchids (Cymbidium spp.) exhibit significant variations in floral morphology, pollinator relations, and ecological habitats. Due to their exceptional economic and ornamental value, Cymbidium spp. have been commercially cultivated for centuries. SSR markers are extensively used genetic tools for biology identification and population genetics analysis. In this study, nine polymorphic EST-SSR loci were isolated from Cymbidium goeringii using RNA-Seq technology. All nine SSR loci showed transferability in seven other congeneric species, including 51 cultivars. The novel SSR markers detected inter-species gene flow among the Cymbidium species and intra-species sub-division of C. goeringii and C. ensifolium, as revealed by neighborhood-joining and Structure clustering analyses. In this study, we developed nine microsatellites using RNA-Seq technology. These SSR markers aided in detecting potential gene flow among Cymbidium species and identified the intra-species sub-division of C. goeringii and C. ensifolium.
ISSN:1471-2229
1471-2229
DOI:10.1186/s12870-023-04499-y