Recombinant adenovirus expressing vesicular stomatitis virus G proteins induce both humoral and cell-mediated immune responses in mice and goats

• Published in: BMC veterinary research 2021-01, Vol.17 (1), p.36-36, Article 36
• Main Authors: Xue, Xiaojuan, Yu, Zhaorong, Jin, Hongyan, Liang, Lin, Li, Jiayang, Li, Xiaolu, Wang, Yong, Cui, Shangjin, Li, Gang
• Format: Article
• Language: English
• Subjects: Adenoviridae; Adenoviruses; Animals; Antibodies; Antibodies, Neutralizing; Antibodies, Viral; Cytomegalovirus; Deoxyribonucleic acid; DNA; DNA vaccines; DNA virus infections; Female; Foot & mouth disease; Fusion protein; Genes; Genetic aspects; Glycoproteins; Goat Diseases - immunology; Goat Diseases - prevention & control; Goat Diseases - virology; Goats; Health aspects; Immune response; Immune response (cell-mediated); Immune response (humoral); Immune responses; Immunity, Cellular; Immunity, Humoral; Immunization; Immunogenicity; Immunological research; Lymphocytes; Mammals; Membrane Glycoproteins - genetics; Membrane Glycoproteins - immunology; Membrane Glycoproteins - metabolism; Mice; Mice, Inbred BALB C; Prevention; Proteins; Recombinant adenovirus; Serotypes; Stomatitis; Vaccines; Vaccines, Synthetic - immunology; Vesicular Stomatitis - immunology; Vesicular Stomatitis - prevention & control; Vesicular stomatitis Indiana virus - immunology; Vesicular stomatitis New Jersey virus - immunology; Vesicular stomatitis virus; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Viral Envelope Proteins - metabolism; Viral vaccines; Viral Vaccines - immunology; Virulence; Zoonoses
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-020-02740-6

Vesicular stomatitis (VS) is an acute, highly contagious and economically important zoonotic disease caused by the vesicular stomatitis virus (VSV). There is a need for effective and safe stable recombinant vaccine for the control of the disease. The human type 5 replication-defective adenovirus expression vector is a good way to construct recombinant vaccines. Three recombinant adenoviruses (rAd) were successfully constructed that expressed the VSV Indiana serotype glycoprotein (VSV-IN-G), VSV New Jersey serotype glycoprotein (VSV-NJ-G), and the G fusion protein (both serotypes of G [VSV-IN-G-NJ-G]) with potentiality to induce protective immunity. G proteins were successfully expressed with good immunogenicity. The rAds could induce the production of VSV antibodies in mice, and VSV neutralizing antibodies in goats, respectively. The neutralizing antibody titers could reach 1:32 in mice and 1:64 in goats. The rAds induced strong lymphocyte proliferation in mice and goats, which was significantly higher compared to the negative control groups. The three rAds constructed in the study expressed VSV-G proteins and induced both humoral and cellular immune responses in mice and goats. These results lay the foundation for further studies on the use of rAds in vaccines expressing VSV-G.