The NAC transcription factor MdNAC29 negatively regulates drought tolerance in apple

Drought stress is an adverse stimulus that affects agricultural production worldwide. NAC transcription factors are involved in plant development and growth but also play different roles in the abiotic stress response. Here, we isolated the apple gene and investigated its role in regulating drought...

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Bibliographic Details
Published in:Frontiers in plant science 2023-07, Vol.14, p.1173107-1173107
Main Authors: Li, Sen, Jing, Xiuli, Tan, Qiuping, Wen, Binbin, Fu, Xiling, Li, Dongmei, Chen, Xiude, Xiao, Wei, Li, Ling
Format: Article
Language:English
Subjects:
apple
drought tolerance
F-box protein
MdDREB2A
MdNAC29
Plant Science
RNA-seq
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Summary:Drought stress is an adverse stimulus that affects agricultural production worldwide. NAC transcription factors are involved in plant development and growth but also play different roles in the abiotic stress response. Here, we isolated the apple gene and investigated its role in regulating drought tolerance. Subcellular localization experiments showed that was localized to the nucleus and transcription was induced by the PEG treatment. Over-expression of reduced drought tolerance in apple plants, calli, and tobacco, and exhibited higher relative conductivity, malondialdehyde (MDA) content, and lower chlorophyll content under drought stress. The transcriptomic analyses revealed that reduced drought resistance by modulating the expression of photosynthesis and leaf senescence-related genes. The qRT-PCR results showed that overexpression of repressed the expression of drought-resistance genes. Yeast one-hybrid and dual-luciferase assays demonstrated that MdNAC29 directly repressed expression. Moreover, the yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that MdNAC29 interacted with the MdPP2-B10 (F-box protein), which responded to drought stress, and MdPP2-B10 enhanced the repressive effect of MdNAC29 on the transcriptional activity of the . Taken together, our results indicate that is a negative regulator of drought resistance, and provide a theoretical basis for further molecular mechanism research.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2023.1173107