Effect of anaerobic bovine colostrum fermentation on bacteria growth inhibition

• Published in: Ciência rural 2016-12, Vol.46 (12), p.2152-2157
• Main Authors: Saalfeld, Mara Helena, Pereira, Daniela Isabel Brayer, Valente, Júlia de Souza Silveira, Borchardt, Jéssica Lopes, Weissheimer, Christiano Fanck, Gularte, Márcia Arocha, Leite, Fábio Pereira Leivas
• Format: Article
• Language: English
• Subjects: AGRONOMY; Anaerobic processes; Animal health; Antibodies; Bacillus cereus; Bacteria; Brucella abortus; Calves; Colostrum; colostrum silage; Dairy farms; Disease transmission; E coli; Escherichia coli; Fermentation; Food contamination & poisoning; Infectious diseases; Laboratories; Lactic acid; Lactic acid bacteria; Leptospira interrogans; Medical research; Milk; milk substitutes; Mycobacterium bovis; Polyethylene terephthalate; Salmonella; Salmonella Enteritidis; Salmonella Typhimurium; Silage; Staphylococcus aureus
• ISSN: 0103-8478; 1678-4596; 0103-8478; 1678-4596
• DOI: 10.1590/0103-8478cr20160393

Efficient handling programs that provide high quality colostrum in adequate amounts to dairy farm calves are needed to assure their health and survival. Replacers (or milk substitutes) often become necessary when colostrum presents inadequate quality, or in order to break the cycle of infectious disease transmission. In this study we aimed to assess the effect of anaerobic fermentation processing (colostrum silage) on bacterial that represent interest to animal health. Colostrum samples were inoculated with cultures of Brucella abortus , Escherichia coli , Leptospira interrogans serovar Copenhageni , Mycobacterium bovis , Salmonella Enteritidis , Salmonella Typhimurium , Staphylococcus aureus , and Bacillus cereus and then subjected to anaerobic fermentation. On the first day, and every seven days until 30th days after fermentation, the samples were cultured and colony forming units counted. At seven days of fermentation, B. abortus , L. interrogans , and M. bovis were not detected. At 14th days of fermentation, E. coli , S. aureus , S. Enteritidis and S. Typhimurium were no longer detected. However, we were able to detect both lactic acid bacteria and B. cereus until 30th days of fermentation. From this study we suggested that anaerobic fermentation processing can inhibit important bacteria that cause economical losses for the cattle industry. The observations suggested that colostrum silage is a promising form to conserve bovine colostrum. RESUMO: Eficientes programas de manejo que permitem o fornecimento de colostro de alta qualidade e em quantidades adequadas para bezerros são necessários para garantir a sua saúde e sobrevivência. Substitutos do leite tornam-se frequentemente necessários quando o colostro apresenta qualidade inadequada ou a fim de quebrar o ciclo de transmissão de doenças infecciosas. Este estudo teve como objetivo avaliar o efeito da fermentação anaeróbica do colostro bovino (silagem de colostro) sobre bactérias de interesse para a saúde animal. Alíquotas de colostro foram inoculadas com culturas de Brucella abortus , Escherichia coli , Leptospira interrogans serovar Copenhageni , Mycobacterium bovis , Salmonella Enteritidis , Salmonella Typhimurium , Staphylococcus aureus e Bacillus cereus e submetidas ao processo de fermentação anaeróbia. No primeiro dia e a cada sete dias até o 30o dia de fermentação, as alíquotas foram cultivadas e analisadas pela contagem de unidades formadoras de colônias. Aos sete dias de fermentação, unidades