Proteome analysis of alfalfa roots in response to water deficit stress

• Published in: Journal of Integrative Agriculture 2016-06, Vol.15 (6), p.1275-1285
• Main Authors: Rahman, Md Atikur, Yong-Goo, Kim, Iftekhar, Alam, LIU, Gong-she, Hyoshin, Lee, Joo, Lee Jeung, Byung-Hyun, Lee
• Format: Article
• Language: English
• Subjects: alfalfa; proteome; root; water deficit stress; 内向整流钾通道; 抗坏血酸过氧化物酶; 水分亏缺; 紫花苜蓿; 聚丙烯酰胺凝胶电泳; 胁迫; 蛋白质组分; 适应性反应
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/S2095-3119(15)61255-2

To evaluate the response of alfalfa to water deficit （WD） stress, WD-induced candidates were investigated through a proteomic approach. Alfalfa seedlings were exposed to WD stress for 12 and 15 days respectively, followed by 3 days re-watering. Water deficit increased H202 content, lipid peroxidation, DPPH （1,1-diphenyl-2-picrylhydrazyl）-radical scavenging activity, and the free proline level in alfalfa roots. Root proteins were extracted and separated by two-dimentional polyacrylamide gel electrophoresis （2-DE）. A total of 49 WD-responsive proteins were identified in alfalfa roots; 25 proteins were reproducibly found to be up-regulated and 24 were down-regulated. Two proteins, namely cytosolic ascorbate peroxidase （APx2） and putative F-box protein were newly detected on 2-DE maps of WD-treated plants. We identified several proteins including agamous-like 65, albumin b-32, inward rectifying potassium channel, and auxin-independent growth promoter. The identified proteins are involved in a variety of cellular functions including calcium signaling, abacisic acid （ABA） biosynthesis, reactive oxygen species （ROS） regulation, transcription/translation, antioxidant/detoxification/stress defense, energy metabolism, signal transduction, and storage. These results indicate the potential candidates were responsible for adaptive response in alfalfa roots.