Chemical Composition and Cytotoxic Activity of Extracts from Carpesium divaricatum : In Vitro- versus Field-Grown Plants

Carpesium divaricatum Sieb. & Zucc. is a plant species rich in terpenoids of anti-inflammatory and cytotoxic activity, especially germacranolides of potential medicinal value. The present study describes in vitro multiplication of C. divaricatum, analysis of active constituents in the multiple s...

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Bibliographic Details
Published in:Plants (Basel) 2022-10, Vol.11 (21), p.2815
Main Authors: Malarz, Janusz, Galanty, Agnieszka, Stojakowska, Anna
Format: Article
Language:English
Subjects:
Biological activity
Carpesium
Chemical composition
Colon cancer
Constituents
Cytotoxicity
Epithelial cells
Epithelium
Hepatocellular carcinoma
Inflammation
Keratinocytes
Leaves
Liquid chromatography
Melanoma
Metabolites
micropropagation
multiple shoots
Physiology
Plant extracts
Plant species
plant tissue culture
Plantlets
Prostate cancer
sesquiterpene lactones
Shoots
Terpenes
Thidiazuron
Toxicity
Tumor cell lines
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Summary:Carpesium divaricatum Sieb. & Zucc. is a plant species rich in terpenoids of anti-inflammatory and cytotoxic activity, especially germacranolides of potential medicinal value. The present study describes in vitro multiplication of C. divaricatum, analysis of active constituents in the multiple shoots, and assessment of cytotoxic activities of extracts prepared from in vitro- and field-grown plants. The plant extracts were evaluated for cytotoxicity using two melanoma cell lines (HTB140 and A375); human keratinocytes (HaCaT); two colon cancer cell lines (Caco2 and HT29); human hepatocellular carcinoma cells (HepG2); two lines of prostate cancer cells (DU145 and PC3) and prostate epithelial cells (PNT2). Chemical compositions of the assayed extracts were analyzed by HPLC/DAD, in reference to isolated compounds. Maximum of 4.07 ± 1.61 shoots regenerated from a nodal explant of C. divaricatum, cultivated in a liquid MS medium supplemented with thidiazuron (1 μM). In vitro grown shoots and plantlets of C. divaricatum accumulated terpenoids that are known as active constituents of the intact plant. Cytotoxic activity of the extracts prepared from the in vitro cultured plants was like that demonstrated by the extracts prepared from field-grown plants and seemed to be more selective than cytotoxicities of the individual germacranolides.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11212815