Silver diamine fluoride (SDF) used in childhood caries management has potent antifungal activity against oral Candida species

The microbiome of Severe-Early Childhood Caries (S-ECC), is characterized by an ecosystem comprising bacterial and fungal species, with a predominance of Candida species. Hence, an anti-cariogen effective against both bacteria and fungi would be valuable in the management of S-ECC. Here we evaluate...

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Bibliographic Details
Published in:BMC microbiology 2020-04, Vol.20 (1), p.95-95, Article 95
Main Authors: Fakhruddin, Kausar Sadia, Egusa, Hiroshi, Ngo, Hien Chi, Panduwawala, Chamila, Pesee, Siripen, Venkatachalam, Thenmozhi, Samaranayake, Lakshman Perera
Format: Article
Language:English
Subjects:
Antibacterial activity
Antibacterial agents
Antifungal activity
Antifungal agents
Antifungal efficacy
Bacteria
Biofilms
Candida
Candida albicans
Candida krusei
Candida species
Cell walls
Childhood
Children
Dental caries
Dentin caries
Dentistry
Diamines
Ecosystems
Filamentation
Fluconazole
Fluoride treatments
Fluorides
Fungi
Fungicides
Health aspects
Low concentrations
Management
Microbiomes
Severe early childhood caries (S ECC)
Silver diamine fluoride
Species
Underserved populations
Yeast
Yeasts
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Summary:The microbiome of Severe-Early Childhood Caries (S-ECC), is characterized by an ecosystem comprising bacterial and fungal species, with a predominance of Candida species. Hence, an anti-cariogen effective against both bacteria and fungi would be valuable in the management of S-ECC. Here we evaluate the antifungal effect of silver diamine fluoride (SDF) against 35-clinical yeast isolates (Ten-each of C. albicans, C. krusei, C. tropicalis and five C. glabrata strains) from dentinal caries-lesions from S-ECC. Disc-diffusion and time-kill assays as well as MIC and MIC evaluations against therapeutic concentrations confirmed the broad-spectrum anti-candidal potency of SDF. Ultrastructural images revealed morphologic aberrations of yeast-cell walls on exposure to SDF. All C. krusei and C. glabrata isolates were significantly more sensitive to SDF, relative to the standard antifungal fluconazole. Further, SDF appears to effectively abrogate filamentation of C. albicans even at very low concentrations. Our data, for the first time, elucidate the antifungal potency of SDF, in addition to its known antibacterial activity, in the management of S-ECC.
ISSN:1471-2180
1471-2180
DOI:10.1186/s12866-020-01776-w