Ex vivo electrochemical measurement of glutamate release during spinal cord injury

[Display omitted] Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recordin...

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Bibliographic Details
Published in:MethodsX 2019-01, Vol.6, p.1894-1900
Main Authors: Nolan, James K., Nguyen, Tran N.H., Fattah, Mara, Page, Jessica C., Shi, Riyi, Lee, Hyowon
Format: Article
Language:English
Subjects:
Additive manufacturing
Biosensor
Direct ink writing
Electrochemical Glutamate Sensing from Resected Spinal Cord Segment
Excitotoxicity
Implantable
Neuroscience
Rapid prototyping
SCI
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Summary:[Display omitted] Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recording chamber are an established model for traumatic spinal cord injury. We have developed a method to record, with micro-scale printed glutamate biosensors, glutamate release from ex vivo rat spinal cord segments following injury. This protocol would work equally well for similar glutamate biosensors.
ISSN:2215-0161
2215-0161
DOI:10.1016/j.mex.2019.08.008