Virulence Profiles, Phylogenetic Background, and Antibiotic Resistance of Escherichia coli Isolated from Turkeys with Airsacculitis

Avian Pathogenic Escherichia coli (APEC) has been studied for decades because of its economic impact on the poultry industry. Recently, the zoonotic potential of APEC and multidrug-resistant strains have emerged. The aim of this study was to characterize 225 APEC isolated from turkeys presenting air...

Full description

Saved in:
Bibliographic Details
Published in:TheScientificWorld 2014-01, Vol.2014 (2014), p.1-8
Main Authors: Knöbl, Terezinha, Gomes, Cleise Ribeiro, da Silva, Ketrin Cristina, de Oliveira, Mirela Caroline Vilela, de Oliveira, Maria Gabriela Xavier, Cunha, Marcos Paulo Vieira, Moreno, Andrea Micke
Format: Article
Language:English
Subjects:
Animals
Antibiotic resistance
Antibiotics
Antimicrobial agents
Contamination
Deoxyribonucleic acid
DNA
Drug resistance
Drug resistance in microorganisms
Drug Resistance, Microbial - genetics
E coli
Economic impact
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - genetics
Escherichia coli - pathogenicity
Escherichia coli Infections - microbiology
Escherichia coli Proteins - genetics
Food contamination
Genetic aspects
Health aspects
Identification and classification
Phylogenetics
Phylogeny
Poultry
Poultry Diseases - microbiology
Public health
Studies
Toxins
Turkeys
Virulence (Microbiology)
Virulence - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Avian Pathogenic Escherichia coli (APEC) has been studied for decades because of its economic impact on the poultry industry. Recently, the zoonotic potential of APEC and multidrug-resistant strains have emerged. The aim of this study was to characterize 225 APEC isolated from turkeys presenting airsacculitis. The results showed that 92% of strains presented a multidrug-resistance (MDR), and the highest levels of resistance were to sulfamethazine (94%) and tetracycline (83%). Half of these strains were classified in phylogenetic group B2, followed by B1 (28.6%), A (17.1%), and D (4.8%). The prevalence of virulence genes was as follows: salmochelin (iroN, 95%), increased serum survival (iss, 93%), colicin V (cvi/cva, 67%), aerobactin (iucD, 67%), temperature-sensitive haemagglutinin (tsh, 56%), iron-repressible protein (irp2, 51%), invasion brain endothelium (ibeA, 31%), vacuolating autotransporter toxin (vat, 24%), K1 antigen (neuS, 19%), enteroaggregative heat-stable cytotoxin (astA, 17%), and pilus associated with pyelonephritis (papC, 15%). These results demonstrate that the majority of the investigated strains belonged to group B2 and were MDR. These data suggest that turkeys may serve as a reservoir of pathogenic and multidrug-resistance strains, reinforcing the idea that poultry plays a role in the epidemiological chain of ExPEC.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1155/2014/289024