In Vitro Analysis of SARS-CoV-2 Spike Protein and Ivermectin Interaction

The spike (S) protein of SARS-CoV-2 is a molecular target of great interest for developing drug therapies against COVID-19 because S is responsible for the interaction of the virus with the host cell receptor. Currently, there is no outpatient safety treatment for COVID-19 disease. Furthermore, we c...

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Published in:International journal of molecular sciences 2023-11, Vol.24 (22), p.16392
Main Authors: García-Aguilar, Alejandra, Campi-Caballero, Rebeca, Visoso-Carvajal, Giovani, García-Sánchez, José Rubén, Correa-Basurto, José, García-Machorro, Jazmín, Espinosa-Raya, Judith
Format: Article
Language:English
Subjects:
Calibration
COVID-19
COVID-19 vaccines
Drug interactions
FDA approval
Health aspects
Hemodialysis
Infections
interaction
Ivermectin
Ligands
Mortality
Pharmaceutical industry
Production costs
Proteins
RNA polymerase
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
spike protein
thermolysin
Viral infections
Viruses
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Summary:The spike (S) protein of SARS-CoV-2 is a molecular target of great interest for developing drug therapies against COVID-19 because S is responsible for the interaction of the virus with the host cell receptor. Currently, there is no outpatient safety treatment for COVID-19 disease. Furthermore, we consider it of worthy importance to evaluate experimentally the possible interaction of drugs (approved by the Food and Drug Administration) and the S, considering some previously in silico and clinical use. Then, the objective of this study was to demonstrate the in vitro interaction of ivermectin with S. The equilibrium dialysis technique with UV–Vis was performed to obtain the affinity and dissociation constants. In addition, the Drug Affinity Responsive Target Stability (DARTS) technique was used to demonstrate the in vitro interaction of S with ivermectin. The results indicate the interaction between ivermectin and the S with an association and dissociation constant of Ka = 1.22 µM−1 and Kd = 0.81 µM, respectively. The interaction was demonstrated in ratios of 1:50 pmol and 1:100 pmol (S: ivermectin) by the DARTS technique. The results obtained with these two different techniques demonstrate an interaction between S and ivermectin previously explored in silico, suggesting its clinical uses to stop the viral spread among susceptible human hosts.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242216392