V-Set and Immunoglobulin Domain-Containing 1 (VSIG1), Predominantly Expressed in Testicular Germ Cells, Is Dispensable for Spermatogenesis and Male Fertility in Mice

To elucidate the functional role of V-set and immunoglobulin domain-containing 1 (VSIG1) in spermatogenesis and fertilization, we knocked out (KO) VSIG1 in a mouse embryo using CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9) -mediated genome editing...

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Bibliographic Details
Published in:Animals (Basel) 2021-04, Vol.11 (4), p.1037
Main Authors: Jung, Yena, Bang, Hyewon, Kim, Young-Hyun, Park, Na-Eun, Park, Young-Ho, Park, Chaeli, Lee, Sang-Rae, Lee, Jeong-Woong, Song, Bong-Seok, Kim, Ji-Su, Sim, Bo-Woong, Seol, Dong-Won, Wee, Gabbine, Kim, Sunhyung, Kim, Sun-Uk, Kim, Ekyune
Format: Article
Language:English
Subjects:
Antigens
Chromosomes
Complementary DNA
CRISPR
Domains
Embryos
Females
Fertility
fertilization
Genetic testing
Genomes
Germ cells
Immunoglobulins
In vitro fertilization
Infertility
Laboratory animals
Mammals
Organs
Proteins
Reverse transcription
Rodents
Sperm
Spermatogenesis
Testes
Tissues
VSIG1
Weight loss
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Summary:To elucidate the functional role of V-set and immunoglobulin domain-containing 1 (VSIG1) in spermatogenesis and fertilization, we knocked out (KO) VSIG1 in a mouse embryo using CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9) -mediated genome editing. Reverse transcription PCR was performed using cDNA synthesized from VSIG1 KO testis RNA. Although Western blot analysis using a specific antibody to VSIG1 confirmed VSIG1 protein defects in the KO mice, hematoxylin-eosin staining analysis was similar in the KO and wild-type mice. Additionally, computer-assisted sperm analysis and in vitro fertilization experiments were conducted to confirm the activity and fertilization ability of sperm derived from the KO mouse. Mice lacking VSIG1 were viable and had no serious developmental defects. As they got older, the KO mice showed slightly higher weight loss, male mice lacking VSIG1 had functional testes, including normal sperm number and motility, and both male and female mice lacking VSIG1 were fertile. Our results from VSIG1 KO mice suggest that VSIG1 may not play essential roles in spermatogenesis and normal testis development, function, and maintenance. VSIG1 in sperm is dispensable for spermatogenesis and male fertility in mice. As several genes are known to possess slightly different functions depending on the species, the importance and molecular mechanism of VSIG1 in tissues of other species needs further investigation.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani11041037