The effect of low doses of glyphosate on reactive oxygen species production by human granulocytes

Glyphosate is the base of numerous herbicides used widely all over the world. Strong hepato-and nephrotoxicity of high doses of this reagent was reported in laboratory animal studies. In European Union countries the acceptable daily intake for humans is set at 0.5 mg/kg body weight. We investigated...

Full description

Saved in:
Bibliographic Details
Published in:European Journal of Biological Research 2023-10, Vol.13 (3), p.161-172
Main Authors: Sikora, Jacek, Jagielska, Joanna, Kaszkowiak, Krzysztof
Format: Article
Language:English
Subjects:
Acetic acid
Body weight
Glyphosate
Granulocytes
Herbicides
Human peripheral blood polymorphonuclear cells
Kinases
Laboratory animals
Leukocytes (granulocytic)
Leukocytes (polymorphonuclear)
NAD(P)H oxidase
Peripheral blood
Phagocytes
Protein kinase C
Reactive oxygen species
Reagents
Respiratory burst
Statistical analysis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Glyphosate is the base of numerous herbicides used widely all over the world. Strong hepato-and nephrotoxicity of high doses of this reagent was reported in laboratory animal studies. In European Union countries the acceptable daily intake for humans is set at 0.5 mg/kg body weight. We investigated the effects of glyphosate on peripheral blood polymorphonuclear cells (PMNs) at relatively low concentrations of the reagent, from 0.01 mg/L to 10 mg/L (from ~0.06 µM to 59 µM). As the biological half-life of this compound in the human body is estimated to be 3 to 10 hours, we decided to incubate blood samples with glyphosate for a period of one hour. Such incubation caused a statistically significant increase of reactive oxygen species production in granulocytes stimulated with N-formylmethionine-leucyl-phenylalanine and Escherichia coli cells. This increase was not associated with the toxic effects of glyphosate or with increased phagocytic activity of granulocytes. The reagent, when applied at specified concentrations, did not induce a respiratory burst in granulocytes or affect the amount of production of reactive oxygen species in blood samples stimulated with 12-myristate phorbol 13-acetate. On the basis of the results obtained, it may be suggested that glyphosate affects signaling pathways leading to NADPH oxidase activation, independent of protein kinase C activation. Thus, it can be concluded that although low doses of glyphosate are not harmful to humans, synergistic effects of this compound with other environmental pollutants may be an important part of pathogenic mechanisms.
ISSN:2449-8955
DOI:10.5281/zenodo.8428575