Metabolites from Microbes Isolated from the Skin of the Panamanian Rocket Frog Colostethus panamansis (Anura: Dendrobatidae)

The Panamanian rocket frog Colostethus panamansis (family Dendrobatidae) has been affected by chytridiomycosis, a deadly disease caused by the fungus Batrachochytrium dendrobatidis (Bd). While there are still uninfected frogs, we set out to isolate microbes from anatomically distinct regions in an e...

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Bibliographic Details
Published in:Metabolites 2020-10, Vol.10 (10), p.406
Main Authors: Martin H., Christian, Ibáñez, Roberto, Nothias, Louis-Félix, Caraballo-Rodríguez, Andrés Mauricio, Dorrestein, Pieter C., Gutiérrez, Marcelino
Format: Article
Language:English
Subjects:
16S rRNA sequencing
3D molecular cartography
Annotations
Bacteria
Cartography
Chytridiomycosis
Colostethus
Colostethus panamansis
Dendrobatidae
feature-based molecular networking
Frogs
Mass spectrometry
Mass spectroscopy
Metabolites
Microorganisms
Peptides
Scientific imaging
Skin
skin-associated bacteria
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Summary:The Panamanian rocket frog Colostethus panamansis (family Dendrobatidae) has been affected by chytridiomycosis, a deadly disease caused by the fungus Batrachochytrium dendrobatidis (Bd). While there are still uninfected frogs, we set out to isolate microbes from anatomically distinct regions in an effort to create a cultivable resource within Panama for potential drug/agricultural/ecological applications that perhaps could also be used as part of a strategy to protect frogs from infections. To understand if there are specific anatomies that should be explored in future applications of this resource, we mapped skin-associated bacteria of C. panamansis and their metabolite production potential by mass spectrometry on a 3D model. Our results indicate that five bacterial families (Enterobacteriaceae, Comamonadaceae, Aeromonadaceae, Staphylococcaceae and Pseudomonadaceae) dominate the cultivable microbes from the skin of C. panamansis. The combination of microbial classification and molecular analysis in relation to the anti-Bd inhibitory databases reveals the resource has future potential for amphibian conservation.
ISSN:2218-1989
2218-1989
DOI:10.3390/metabo10100406