Distribution, Morphological Characterization, and Resiniferatoxin-Susceptibility of Sensory Neurons That Innervate Rat Perirenal Adipose Tissue

Perirenal adipose tissue (PrAT) is a visceral adipose tissue involved in the pathogenesis of obesity and cardiovascular diseases via neural pathways. However, the origins, morphological characterization, and resiniferatoxin (RTX)-susceptibility of sensory neurons that innervate rat PrAT are yet uncl...

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Published in:Frontiers in neuroanatomy 2019-03, Vol.13, p.29-29
Main Authors: Liu, Bo-Xun, Qiu, Ming, Zong, Peng-Yu, Chen, Xu-Guan, Zhao, Kun, Li, Yong, Li, Peng, Sun, Wei, Kong, Xiang-Qing
Format: Article
Language:English
Subjects:
Abdomen
Adipose tissue
Body fat
Capsaicin receptors
Cardiovascular disease
Cardiovascular diseases
Denervation
Dorsal root ganglia
Hypertension
Immunofluorescence
Injection
Innervation
Laboratory animals
Morphology
neural tracing
Neurons
Neuroscience
Neurosciences
Obesity
Pathogenesis
Perchloric acid
perirenal adipose tissue
Resiniferatoxin
Rodents
Sensory neurons
Transient receptor potential proteins
TRPV1
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Summary:Perirenal adipose tissue (PrAT) is a visceral adipose tissue involved in the pathogenesis of obesity and cardiovascular diseases via neural pathways. However, the origins, morphological characterization, and resiniferatoxin (RTX)-susceptibility of sensory neurons that innervate rat PrAT are yet unclear. Using neural tracing, an injection of DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) into PrAT revealed that sensory neurons that innervate PrAT reside in T9-L3 dorsal root ganglia (DRG). Peak labeling occurred in T13 and L1 DRGs. Two distinct peaks were observed in cross-sectional areas of the labeled soma, and the mean cross-sectional area was 717.1 ± 27.7 μm2. Immunofluorescence staining for transient receptor potential cation channel subfamily V member 1 (TRPV1) separated DiI-positive neurons into three subpopulations: small TRPV1-negative, small TRPV1-positive, and large TRPV1-negative. Furthermore, the injection of RTX into PrAT reduced labeled cells by 36.7% where TRPV1-positive cells were the main target of RTX denervation. These novel findings provide a structural basis for future TRPV1-dependent and TRPV1-independent studies on the sensory innervation of PrAT, which may be of interest for future therapeutic obesity treatment and intervention.
ISSN:1662-5129
1662-5129
DOI:10.3389/fnana.2019.00029