Study of the Effect of Baicalin from Scutellaria baicalensis on the Gastrointestinal Tract Normoflora and Helicobacter pylori

• Published in: International journal of molecular sciences 2023-07, Vol.24 (15), p.11906
• Main Authors: Dmitrieva, Anastasia, Kozlova, Oksana, Atuchin, Victor, Milentieva, Irina, Vesnina, Anna, Ivanova, Svetlana, Asyakina, Lyudmila, Prosekov, Alexander
• Format: Article
• Language: English
• Subjects: Antibiotics; antimicrobial activity; Antimicrobial agents; baicalin; Consumers; Ethanol; Fatty acids; Flavonoids; Genes; Helicobacter pylori; Herbal medicine; lactic acid bacteria; Microbiota; Microorganisms; Peptides; Prebiotics; Probiotics; Scutellaria baicalensis; symbiosis; Virulence
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241511906

The antimicrobial properties of baicalin against and several probiotic cultures were evaluated. Baicalin was isolated from a dry plant extract obtained by extraction with water at 70 °C. For isolation, extraction was carried out with n-butanol and purification on a chromatographic column. The antimicrobial potential was assessed by evaluating changes in the optical density of the bacterial suspension during cultivation; additionally, the disk diffusion method was used. During the study, the baicalin concentrations (0.25, 0.5, and 1 mg/mL) and the pH of the medium in the range of 1.5-8.0 were tested. The test objects were: suspensions of , , , , and . It was found that the greater the concentration of the substance in the solution, the greater the delay in the growth of the strain zone. Thus, the highest antimicrobial activity against was observed at pH 1.5-2.0 and a baicalin concentration of 1.00 mg/mL. In relation to probiotic strains, a stimulating effect of baicalin (1.00 mg/mL) on the growth of biomass at pH 1.5-2.0 was observed. The results open up the prospects for the use of baicalin and probiotics for the treatment of diseases caused by .