Distinct submembrane localisation compartmentalises cardiac NPR1 and NPR2 signalling to cGMP

• Published in: Nature communications 2018-06, Vol.9 (1), p.2446-9, Article 2446
• Main Authors: Subramanian, Hariharan, Froese, Alexander, Jönsson, Peter, Schmidt, Hannes, Gorelik, Julia, Nikolaev, Viacheslav O.
• Format: Article
• Language: English
• Subjects: 14/33; 14/63; 147/136; 631/57/2282; 631/80/2373; 64/60; 9/10; 96/63; Animals; Atrial Natriuretic Factor - metabolism; Biosensing Techniques; Biosensors; Cardiac and Cardiovascular Systems; Cardiomyocytes; Cell Membrane - metabolism; Cell Membrane - ultrastructure; Clinical Medicine; Conductance; Cyclic GMP; Cyclic GMP - metabolism; Cyclic Nucleotide Phosphodiesterases, Type 2 - antagonists & inhibitors; Cyclic Nucleotide Phosphodiesterases, Type 2 - metabolism; Echocardiography; Energy transfer; Experiments; Fluorescence Resonance Energy Transfer; Guanosine; Heart; Heart - diagnostic imaging; HEK293 Cells; Hormones; Humanities and Social Sciences; Humans; Hypertrophy; Imidazoles - pharmacology; Invaginations; Kardiologi; Klinisk medicin; Ligands; Localization; Medical and Health Sciences; Medical research; Medicin och hälsovetenskap; Mice; Mice, Knockout; Microscopy; Microscopy, Scanning Probe; Molecular chains; multidisciplinary; Muscle contraction; Myocardium - cytology; Myocardium - metabolism; Myocytes, Cardiac - cytology; Myocytes, Cardiac - metabolism; Natriuretic Peptide, C-Type - metabolism; Peptides; Phosphodiesterase; Primary Cell Culture; Receptors; Receptors, Atrial Natriuretic Factor - genetics; Receptors, Atrial Natriuretic Factor - metabolism; Science; Science (multidisciplinary); Signal Transduction - drug effects; Triazines - pharmacology; Tubules
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-018-04891-5

Natriuretic peptides (NPs) are important hormones that regulate multiple cellular functions including cardiovascular physiology. In the heart, two natriuretic peptide receptors NPR1 and NPR2 act as membrane guanylyl cyclases to produce 3′,5′-cyclic guanosine monophosphate (cGMP). Although both receptors protect from cardiac hypertrophy, their effects on contractility are markedly different, from little effect (NPR1) to pronounced negative inotropic and positive lusitropic responses (NPR2) with unclear underlying mechanisms. Here we use a scanning ion conductance microscopy (SICM) approach combined with Förster resonance energy transfer (FRET)-based cGMP biosensors to show that whereas NPR2 is uniformly localised on the cardiomyocyte membrane, functional NPR1 receptors are found exclusively in membrane invaginations called transverse (T)-tubules. This leads to far-reaching CNP/NPR2/cGMP signals, whereas ANP/NPR1/cGMP signals are highly confined to T-tubular microdomains by local pools of phosphodiesterase 2. This provides a previously unrecognised molecular basis for clearly distinct functional effects engaged by different cGMP producing membrane receptors. Natriuretic peptides (NPs) are important hormones that regulate cardiovascular physiology by increasing cGMP levels in cardiomyocytes. Here the authors use scanning ion conductance microscopy and a cGMP FRET sensor to identify a differential localisation pattern for the natriuretic peptide receptors within the heart.