Concurrent micro- to macro-cardiac electrophysiology in myocyte cultures and human heart slices

The contact cardiac electrogram is derived from the extracellular manifestation of cellular action potentials and cell-to-cell communication. It is used to guide catheter based clinical procedures. Theoretically, the contact electrogram and the cellular action potential are directly related, and sho...

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Bibliographic Details
Published in:Scientific reports 2018-05, Vol.8 (1), p.6947-13, Article 6947
Main Authors: Chowdhury, Rasheda A., Tzortzis, Konstantinos N., Dupont, Emmanuel, Selvadurai, Shaun, Perbellini, Filippo, Cantwell, Chris D., Ng, Fu Siong, Simon, Andre R., Terracciano, Cesare M., Peters, Nicholas S.
Format: Article
Language:English
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Ablation
Action potential
Action Potentials
Cameras
Cardiac Electrophysiology
Catheters
Cell interactions
Cells, Cultured
Electric fields
Electrocardiography
Electrodes
Electrophysiological Phenomena
Electrophysiology
Heart
Heart - physiology
Heart diseases
Humanities and Social Sciences
Humans
Medical instruments
Microelectrodes
Morphology
multidisciplinary
Myocardium - cytology
Myocardium - metabolism
Myocytes, Cardiac - physiology
Science
Science (multidisciplinary)
Stem cells
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Summary:The contact cardiac electrogram is derived from the extracellular manifestation of cellular action potentials and cell-to-cell communication. It is used to guide catheter based clinical procedures. Theoretically, the contact electrogram and the cellular action potential are directly related, and should change in conjunction with each other during arrhythmogenesis, however there is currently no methodology by which to concurrently record both electrograms and action potentials in the same preparation for direct validation of their relationships and their direct mechanistic links. We report a novel dual modality apparatus for concurrent electrogram and cellular action potential recording at a single cell level within multicellular preparations. We further demonstrate the capabilities of this system to validate the direct link between these two modalities of voltage recordings.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-25170-9