Simultaneous improvement of lincomycin A production and reduction of lincomycin B levels in Streptomyces lincolnensis using a combined medium optimization approach

The current study aimed to optimize the culture and production parameters of industrial production of lincomycin A by Streptomyces lincolnensis using a statistical approach that could also reduce unwanted by-products. The Plackett-Burman design, steepest ascent method, and response surface design we...

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Bibliographic Details
Published in:Annals of microbiology 2022-04, Vol.72 (1), p.16-16, Article 16
Main Authors: Zheng, Xiu-hong, Ye, Rui-fang, Ding, Qi-hang, Hu, Feng-xian, Zhang, Hong-zhou, Lai, Shen
Format: Article
Language:English
Subjects:
Antibacterial agents
Antibiotics
Bacteria
Biosynthesis
Byproducts
Cell growth
Fermentation
Genes
Genetic engineering
Glucose
Gram-positive bacteria
Lincomycin
Liquor
Medium optimization
Methods
Nitrogen
Optimization
Osmosis
Osmotic stress
qRT-PCR
quantitative polymerase chain reaction
statistical analysis
Streptomyces
Streptomyces lincolnensis
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Summary:The current study aimed to optimize the culture and production parameters of industrial production of lincomycin A by Streptomyces lincolnensis using a statistical approach that could also reduce unwanted by-products. The Plackett-Burman design, steepest ascent method, and response surface design were used to evaluate different factors that affect lincomycin A production. Using an optimized S. lincolnensis fermentation medium, lincomycin A production was increased up to 4600 mg/L in shaking flasks, which indicated a 28.3% improvement over previous production in an un-optimized medium (3585 mg/L). Additionally, the concentration of lincomycin B by-product was reduced to 0.8%, which was 82.2% lower than that in the un-optimized medium. Further, quantitative real-time PCR analysis revealed the optimized medium improved lincomycin A production by stimulating key genes in the lincomycin A biosynthesis pathway, as well as an osmotic stress gene. Based on the results, the sequential optimization strategy in this study provides powerful means for the enhancement of lincomycin A with less by-product. We found that osmotic stress reduced the concentration of lincomycin B, which could also help reduce fermentation by-product yields in other actinobacteria.
ISSN:1590-4261
1869-2044
DOI:10.1186/s13213-022-01672-w