Method Development and Validation for Simultaneous Analysis of Eleven Components for Quality Control of Geumgwesingihwan Using HPLC–DAD and UPLC–MS/MS

Geumgwesingihwan (GGSGH) is an oriental herbal formula made by adding Achyranthes bidentate Blume and Plantago asiatica L. to Yukmijiwhanghwan. It has been used for the treatment of edema since ancient times. The purpose of this study is to develop and validate a method for simultaneous quantificati...

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Bibliographic Details
Published in:Separations 2022-08, Vol.9 (8), p.213
Main Authors: Seo, Chang-Seob, Lee, Mee-Young
Format: Article
Language:English
Subjects:
Acetic acid
Acetonitrile
Acids
Benzoic acid
Chromatography
Distilled water
Edema
Formic acid
Gallic acid
Geumgwesingihwan
Herbal medicine
High performance liquid chromatography
Hydroxymethylfurfural
Manufacturing
Mass spectrometry
Medicine
Medicine, Botanic
Medicine, Herbal
Methods
Organic acids
Phytochemicals
Quality assessment
Quality control
Scientific imaging
simultaneous analysis
Steroids
ultra-performance liquid chromatography–tandem mass spectrometry
validation
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Summary:Geumgwesingihwan (GGSGH) is an oriental herbal formula made by adding Achyranthes bidentate Blume and Plantago asiatica L. to Yukmijiwhanghwan. It has been used for the treatment of edema since ancient times. The purpose of this study is to develop and validate a method for simultaneous quantification of 11 components: gallic acid (1), 5-(hydroxymethyl)furfural (2), geniposidic acid (3), morroniside (4), loganin (5), paeoniflorin (6), acteoside (7), cornuside (8), benzoic acid (9), benzoylpaeoniflorin (10), and paeonol (11), using high-performance liquid chromatography with a diode array detector (HPLC–DAD) and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). Compounds 1–11 were separated on a Capcell Pak UG 80 C18 column (250 mm × 4.6 mm, 5 μm) using a mobile phase of a distilled water–acetonitrile system, both containing 0.1% formic acid. In UPLC–MS/MS, compounds 1–11 were separated on an Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 µm) using a mobile phase of a distilled water–acetonitrile system containing 1.0% acetic acid. Using these methods, samples of GGSGH were determined to contain 0.13–2.87 mg/g (HPLC–DAD) and not detected–4.60 mg/g (UPLC–MS/MS) of compounds 1–11. The developed HPLC–DAD assays for simultaneous determination of all analytes were validated with respect to linearity, limits of detection and quantification, recovery, and precision. The established HPLC assay will be used to obtain basic data for quality evaluation of GGSGH and related oriental herbal formulas.
ISSN:2297-8739
2297-8739
DOI:10.3390/separations9080213