Effects of irisin on the dysfunction of blood–brain barrier in rats after focal cerebral ischemia/reperfusion

Objective To investigate whether irisin could protect against blood–brain barrier (BBB) dysfunction following focal cerebral ischemia/reperfusion in rats. Methods and Materials Seventy‐two adult male Sprague Dawley rats weighing 280–320 g were randomly divided into three groups: sham operation group...

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Bibliographic Details
Published in:Brain and behavior 2019-10, Vol.9 (10), p.e01425-n/a
Main Authors: Guo, Peipei, Jin, Zhao, Wu, Huisheng, Li, Xinyi, Ke, Jianjuan, Zhang, Zongze, Zhao, Qiu
Format: Article
Language:English
Subjects:
Animal cognition
Animals
Apoptosis
Blood-brain barrier
Blood-Brain Barrier - drug effects
Blood-Brain Barrier - metabolism
Blood-Brain Barrier - physiopathology
Brain damage
Brain Ischemia - metabolism
Brain Ischemia - physiopathology
Brain Ischemia - therapy
Carotid arteries
cerebral ischemia/reperfusion
Cognitive ability
Edema
Fibronectins - metabolism
Fibronectins - pharmacology
Infarction, Middle Cerebral Artery - metabolism
Infarction, Middle Cerebral Artery - physiopathology
Infarction, Middle Cerebral Artery - therapy
irisin
Ischemia
Laboratory animals
Male
matrix metalloproteinase‐9
Metabolism
Mortality
Original Research
Permeability
Protective Agents - metabolism
Protective Agents - pharmacology
Rats
Rats, Sprague-Dawley
Stroke
Studies
Treatment Outcome
Veins & arteries
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Summary:Objective To investigate whether irisin could protect against blood–brain barrier (BBB) dysfunction following focal cerebral ischemia/reperfusion in rats. Methods and Materials Seventy‐two adult male Sprague Dawley rats weighing 280–320 g were randomly divided into three groups: sham operation group (S), focal cerebral ischemia/reperfusion group (FC), and irisin group (IR). Focal cerebral ischemia was induced by improved thread occlusion of right middle cerebral artery (MCAO) for 2 hr followed by reperfusion for 24 hr in rats. After 24 hr of reperfusion, the neurological evaluation was performed by the method of Longa's score. The histopathological changes were observed by HE staining. The brain water content was determined by detecting the wet weight and dry weight. The BBB permeability was assessed by fluorescence spectrophotometer and fluorescence microscopy for Evans blue (EB) extravasation. The activity and expression of matrix metalloproteinase‐9 (MMP‐9) in different groups were detected by immunohistochemical staining, Western blot, and gel gelatin zymography. Results After MCAO, the neurological deficit scores, the infarct volume, the brain water content, and the EB content were higher in the FC group than those in the S group (p 
ISSN:2162-3279
2162-3279
DOI:10.1002/brb3.1425