NADP-malic Enzyme OsNADP-ME2 Modulates Plant Height Involving in Gibberellin Signaling in Rice

• Published in: Rice (New York, N.Y.) N.Y.), 2024-12, Vol.17 (1), p.52-10
• Main Authors: Li, Bing, Zhou, Xiaolong, Yao, Wei, Lin, Jinjun, Ding, Xiaowen, Chen, Qianru, Huang, Hao, Chen, Wenfeng, Huang, Xilai, Pan, Sujun, Xiao, Yinghui, Liu, Jianfeng, Liu, Xionglun, Liu, Jinling
• Format: Article
• Language: English
• Subjects: Accumulation; Agriculture; Biological activity; Biomedical and Life Sciences; Biosynthesis; Enzymes; Gene expression; Genes; Genetic resources; Genotype & phenotype; Gibberellin signaling; Gibberellins; Life Sciences; Localization; Malic acid; Malic enzyme; Metabolism; NADP; NADP-malic enzyme; OsNADP-ME2; Oxidative metabolism; Pathogens; Phenotypes; Plant Breeding/Biotechnology; Plant Ecology; Plant Genetics and Genomics; Plant growth; Plant height; Plant hormones; Plant Sciences; Plant structures; Proteins; Pyruvic acid; Rice; Rice (Oryza sativa L.); RNA-mediated interference; Seeds; Signal transduction
• ISSN: 1939-8425; 1939-8433; 1934-8037
• DOI: 10.1186/s12284-024-00729-5

Plants NADP-malic enzymes (NADP-MEs) act as a class of oxidative decarboxylase to mediate malic acid metabolism in organisms. Despite NADP-MEs have been demonstrated to play pivotal roles in regulating diverse biological processes, the role of NADP-MEs involving in plant growth and development remains rarely known. Here, we characterized the function of rice cytosolic OsNADP-ME2 in regulating plant height. The results showed that RNAi silencing and knock-out of OsNADP-ME2 in rice results in a dwarf plant structure, associating with significant expression inhibition of genes involving in phytohormone Gibberellin (GA) biosynthesis and signaling transduction, but with up-regulation for the expression of GA signaling suppressor SLR1 . The accumulation of major bioactive GA 1 , GA 4 and GA 7 are evidently altered in RNAi lines, and exogenous GA treatment compromises the dwarf phenotype of OsNADP-ME2 RNAi lines. RNAi silencing of OsNADP-ME2 also causes the reduction of NADP-ME activity associating with decreased production of pyruvate. Thus, our data revealed a novel function of plant NADP-MEs in modulation of rice plant height through regulating bioactive GAs accumulation and GA signaling, and provided a valuable gene resource for rice plant architecture improvement.