Probing interneuronal cell communication via optogenetic stimulation

This study uses an all‐optical approach to probe interneuronal communication between spiral ganglion neurons (SGNs) and neurons of other functional units, in this case cortex neurons (CNs) and hippocampus neurons (HNs), for the first time. We combined a channelrhodopsin variant (CheRiff) with a red...

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Bibliographic Details
Published in:Translational biophotonics 2021-08, Vol.3 (3), p.n/a
Main Authors: Heeger, Patrick, Harre, Jennifer, Warnecke, Athanasia, Mueller, Dominik, Kalies, Stefan, Heisterkamp, Alexander
Format: Article
Language:English
Subjects:
Calcium
cell communication
Cell interactions
Communication
Gene expression
Genetic code
Hearing loss
light
Neurological diseases
Neurons
neuroprosthesis
optogenetics
Population
Pulse duration
Spiral ganglion
Stimulation
Time series
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Summary:This study uses an all‐optical approach to probe interneuronal communication between spiral ganglion neurons (SGNs) and neurons of other functional units, in this case cortex neurons (CNs) and hippocampus neurons (HNs), for the first time. We combined a channelrhodopsin variant (CheRiff) with a red genetically encoded calcium indicator (jRCaMP1a), enabling simultaneous optical stimulation and recording from spatially separated small neuronal populations. Stimulation of SGNs was possible with both optogenetic manipulated HNs and CNs, respectively. Furthermore, a dependency on the pulse duration of the stimulating light in regard to the evoked calcium response in the SGNs was also observed. Our results pave the way to enable innovative technologies based on “biohybrid” systems utilizing the functional interaction between different biological (eg, neural) systems. This can enable improved treatment of neurological and sensorineural disorders such as hearing loss. This study uses an all‐optical approach to probe interneuronal communication between spiral ganglion neurons (SGNs) and neurons of other functional units, in this case cortex neurons (CNs) and hippocampus neurons (HNs), for the first time. We combined a channelrhodopsin variant (CheRiff) with a red genetically encoded calcium indicator (jRCaMP1a), enabling simultaneous optical stimulation and recording from spatially separated small neuronal populations. Our results pave the way to enable innovative technologies based on “biohybrid” systems utilizing the functional interaction between different biological (eg, neural) systems.
ISSN:2627-1850
2627-1850
DOI:10.1002/tbio.202100002