TIPE2 inhibits melanoma progression through MEK/ERK signaling

Recent studies have uncovered that TIPE2 is involved in the development of cancer. However, less research has been conducted on the role of TIPE2 in melanoma. Our study aims to elucidate the mechanism of action of TIPE2 in the development of melanoma. We examined TIPE2 expression in paracarcinoma ti...

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Bibliographic Details
Published in:Scientific reports 2024-11, Vol.14 (1), p.27736-10, Article 27736
Main Authors: Gao, Xin, Li, Yan, Wang, Congcong, Zhao, Guowei
Format: Article
Language:English
Subjects:
692/4028/67/1813/1634
692/53/2421
Animals
Cell Line, Tumor
Cell migration
Cell Movement
Cell Proliferation
Disease Progression
Drug development
ERK
Female
Gene Expression Regulation, Neoplastic
Humanities and Social Sciences
Humans
Immunohistochemistry
Intracellular Signaling Peptides and Proteins - genetics
Intracellular Signaling Peptides and Proteins - metabolism
Male
MAP Kinase Signaling System
Melanoma
Melanoma - genetics
Melanoma - metabolism
Melanoma - pathology
Mice
Mice, Nude
Migration
multidisciplinary
Phosphorylation
Proliferation
Science
Science (multidisciplinary)
Therapeutic targets
TIPE2
Tumors
Wound healing
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Summary:Recent studies have uncovered that TIPE2 is involved in the development of cancer. However, less research has been conducted on the role of TIPE2 in melanoma. Our study aims to elucidate the mechanism of action of TIPE2 in the development of melanoma. We examined TIPE2 expression in paracarcinoma tissue and melanoma tissues and found that TIPE2 expression was downregulated in melanoma tissue compared with paracarcinoma tissue. Overexpression of TIPE2 significantly inhibited the proliferation of melanoma cells in vitro and even inhibited tumor formation in vivo. The CCK8 assay results indicated that TIPE2 overexpression suppressed the proliferation of melanoma cells. The colony-forming ability and wound healing ability of TIPE2-overexpressing melanoma cells were significantly reduced compared with those of control cells. Moreover, immunohistochemistry experiments using a nude mouse tumor model showed consistent results. TIPE2 inhibited the phosphorylation of MEK and ERK. In summary, TIPE2 suppresses the proliferation and migration of melanoma cells by affecting proliferation-related factors and possibly by regulating the MEK/ERK pathway. TIPE2 could be used to inhibit melanoma growth and is a potential drug target for future drug development.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-76794-z