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Screening and characterization of aptamers recognizing the periodontal pathogen Tannerella forsythia
Periodontal disease is one of the most common forms of inflammation. It is currently diagnosed by observing symptoms such as gingival bleeding and attachment loss. However, the detection of biomarkers that precede such symptoms would allow earlier diagnosis and prevention. Aptamers are short oligonu...
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Published in: | FEBS open bio 2024-03, Vol.14 (3), p.498-504 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | Periodontal disease is one of the most common forms of inflammation. It is currently diagnosed by observing symptoms such as gingival bleeding and attachment loss. However, the detection of biomarkers that precede such symptoms would allow earlier diagnosis and prevention. Aptamers are short oligonucleotides or peptides that fold into three‐dimensional conformations conferring the ability to bind molecular targets with high affinity and specificity. Here we report the selection of aptamers that bind specifically to the bacterium Tannerella forsythia, a pathogen frequently associated with periodontal disease. Two aptamers with the highest affinity were examined in more detail, revealing that their binding is probably dependent on mirolysin, a surface‐associated protease secreted by the T. forsythia type‐9 secretion system. The aptamers showed minimal cross‐reactivity to other periodontopathogens and are therefore promising leads for the development of new tools to study the composition of the periodontitis‐associated dysbiotic bacteriome as well as inexpensive new diagnostic assays.
In this study, we identified aptamers with high specificity for the periodontal pathogen Tannerella forsythia, commonly linked to periodontal disease. Two top‐affinity aptamers were further investigated, demonstrating minimal cross‐reactivity with other periodontopathogens. These aptamers hold potential for developing novel tools to explore the composition of the dysbiotic bacteriome in periodontitis and cost‐effective diagnostic assays. |
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ISSN: | 2211-5463 2211-5463 |
DOI: | 10.1002/2211-5463.13772 |