Surfactin and Spo0A-Dependent Antagonism by Bacillus subtilis Strain UD1022 against Medicago sativa Phytopathogens

Plant growth-promoting rhizobacteria (PGPR) such as the root colonizers spp. may be ideal alternatives to chemical crop treatments. This work sought to extend the application of the broadly active PGPR UD1022 to (alfalfa). Alfalfa is susceptible to many phytopathogens resulting in losses of crop yie...

Full description

Saved in:
Bibliographic Details
Published in:Plants (Basel) 2023-02, Vol.12 (5), p.1007
Main Authors: Rosier, Amanda, Pomerleau, Maude, Beauregard, Pascale B, Samac, Deborah A, Bais, Harsh P
Format: Article
Language:English
Subjects:
Alfalfa
antagonism
Antibiotics
Ascochyta medicaginicola
Bacillus subtilis
Biofilms
Crop yield
Fusarium oxysporum
Medicago sativa
Metabolites
Nutrient loss
Nutritive value
Pathogens
PGPR
Phytophthora medicaginis
Plant growth
Surfactin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plant growth-promoting rhizobacteria (PGPR) such as the root colonizers spp. may be ideal alternatives to chemical crop treatments. This work sought to extend the application of the broadly active PGPR UD1022 to (alfalfa). Alfalfa is susceptible to many phytopathogens resulting in losses of crop yield and nutrient value. UD1022 was cocultured with four alfalfa pathogen strains to test antagonism. We found UD1022 to be directly antagonistic toward , (formerly ), and , and not toward f. sp. . Using mutant UD1022 strains lacking genes in the nonribosomal peptide (NRP) and biofilm pathways, we tested antagonism against StC 306-5 and A2A1. The NRP surfactin may have a role in the antagonism toward the ascomycete StC 306-5. Antagonism toward A2A1 may be influenced by biofilm pathway components. The central regulator of both surfactin and biofilm pathways Spo0A was required for the antagonism of both phytopathogens. The results of this study indicate that the PGPR UD1022 would be a good candidate for further investigations into its antagonistic activities against , , and in plant and field studies.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants12051007