Placental Sampling for Understanding Viral Infections - A Simplified Protocol for the COVID-19 Pandemic

The coronavirus disease 2019 (COVID-19) is a pandemic viral disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The impact of the disease among the obstetric population remains unclear, and the study of the placenta can provide valuable information. Adequate sampling of...

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Bibliographic Details
Published in:Revista Brasileira de ginecologia e obstetrícia 2021-05, Vol.43 (5), p.377-383
Main Authors: Nobrega, Guilherme de Moraes, Guida, José Paulo Siqueira, Japecanga, Rodolfo Rosa, Antolini-Tavares, Arthur, Mysorekar, Indira, Costa, Maria Laura
Format: Article
Language:English
Subjects:
COVID-19
COVID-19 - virology
Female
Humans
OBSTETRICS & GYNECOLOGY
Original
placenta
Placenta - virology
Pregnancy
Specimen Handling - methods
Specimen Handling - standards
systematic sampling
viral infections
Virology - methods
Virology - standards
Virus Diseases - virology
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Summary:The coronavirus disease 2019 (COVID-19) is a pandemic viral disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The impact of the disease among the obstetric population remains unclear, and the study of the placenta can provide valuable information. Adequate sampling of the placental tissue can help characterize the pathways of viral infections.  A protocol of placental sampling is proposed, aiming at guaranteeing representativity of the placenta and describing the adequate conservation of samples and their integrity for future analysis. The protocol is presented in its complete and simplified versions, allowing its implementation in different complexity settings.  Sampling with the minimum possible interval from childbirth is the key for adequate sampling and storage. This protocol has already been implemented during the Zika virus outbreak.  A protocol for adequate sampling and storage of placental tissue is fundamental for adequate evaluation of viral infections on the placenta. During the COVID-19 pandemic, implementation of this protocol may help to elucidate critical aspects of the SARS-CoV-2 infection.
ISSN:0100-7203
1806-9339
1806-9339
DOI:10.1055/s-0041-1729146