MicroRNA miR-263b-5p Regulates Developmental Growth and Cell Association by Suppressing Laminin A in Drosophila

Basement membranes (BMs) play important roles under various physiological conditions in animals, including ecdysozoans. During development, BMs undergo alterations through diverse intrinsic and extrinsic regulatory mechanisms; however, the full complement of pathways controlling these changes remain...

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Published in:Biology (Basel, Switzerland) Switzerland), 2023-08, Vol.12 (8), p.1096
Main Authors: Kim, Chae Jeong, Kim, Hyun Ho, Kim, Hee Kyung, Lee, Sojeong, Jang, Daegyu, Kim, Chanhyeok, Lim, Do-Hwan
Format: Article
Language:English
Subjects:
Adipocytes
basement membrane
Basement membranes
Cell proliferation
Cell size
Drosophila
Eggs
Extracellular matrix
Fat body
Genes
Genotype & phenotype
Insects
Laminin
Laminin A
Metamorphosis
MicroRNAs
miR-263b
miRNA
Proteins
RNA-mediated interference
tissue remodeling
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Summary:Basement membranes (BMs) play important roles under various physiological conditions in animals, including ecdysozoans. During development, BMs undergo alterations through diverse intrinsic and extrinsic regulatory mechanisms; however, the full complement of pathways controlling these changes remain unclear. Here, we found that fat body-overexpression of Drosophila miR-263b, which is highly expressed during the larval-to-pupal transition, resulted in a decrease in the overall size of the larval fat body, and ultimately, in a severe growth defect accompanied by a reduction in cell proliferation and cell size. Interestingly, we further observed that a large proportion of the larval fat body cells were prematurely disassociated from each other. Moreover, we present evidence that miR-263b-5p suppresses the main component of BMs, Laminin A (LanA). Through experiments using RNA interference (RNAi) of LanA, we found that its depletion phenocopied the effects in miR-263b-overexpressing flies. Overall, our findings suggest a potential role for miR-263b in developmental growth and cell association by suppressing LanA expression in the Drosophila fat body.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology12081096